TA-01 Chemical Properties

Boiling point:

511.1±50.0 °C(Predicted)

Density 

1.337±0.06 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

DMF: 5 mg/ml; DMF:PBS(pH 7.2)(1:6): 0.14 mg/ml; DMSO: 3 mg/ml

form 

A crystalline solid

pka

9.26±0.24(Predicted)

color 

White to off-white

TA-01 Usage And Synthesis

Description

TA-01 is a derivative of the p38 MAPK inhibitor SB 203580 (Item Nos. 13067 | 13344) and an inducer of cardiomyocyte differentiation. It is cardiomyogenic at concentrations less than 5 μM, increasing expression of the cardiomyocyte marker NKX2-5 by 2.2-fold and decreasing expression of mesoderm markers and the pre-cardiac marker Isl-1 in HES-3 NKX2-5^eGFP/w cells when applied after embryoid body formation. TA-01 inhibits casein kinase 1ε (CK1ε) and CK1Δ and reduces expression of Wnt/β-catenin signaling pathway members, activities that positively correlate with its cardiomyogenic activity.

Uses

TA 01 induces cardiomyocyte differentiation of pluripotent stem cells.

IC 50

CKIδ: 6.8 nM (IC₅₀); p38 MAP kinase: 6.7 nM (IC₅₀)

References

[1] FILIP LACO . Cardiomyocyte differentiation of pluripotent stem cells with SB203580 analogues correlates with Wnt pathway CK1 inhibition independent of p38 MAPK signaling[J]. Journal of molecular and cellular cardiology, 2015, 80: Pages 56-70. DOI: 10.1016/j.yjmcc.2014.12.003

TA-01(1784751-18-3)Related Product Information

• TA-02