anti-HIS Tag Rabbit Polyclonal antibody Usage And Synthesis

Description

Monoclonal antibodies are produced by immunizing mouse with synthesized polypeptide 6 x His. This antibody was purified via protein-A affinity chromatography.

Source

Rabbit

Applications

ELISA and W.B. The researcher should titrate to obtain optimal results.

Reactivity

All

Background

His tag peptide also called polyhistidine-tag is chain of amino acids that are attached to proteins and consist of at least six histidine (6xHis, Hexa Histidine) residues, often at the N- or C-terminus of the protein. Polyhistidine-tags are used for affinity purification of genetically engineered proteins.

References

[1] Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53.
[2] di Guan, C. et al. (1988) Gene 67, 21-30.