ANTI-AKT1 Chemical Properties

storage temp. 

-20°C

form 

buffered aqueous solution

biological source

rabbit

Safety Information

Hazard Codes 

Xn

Risk Statements 

21/22

Safety Statements 

36/37

WGK Germany 

WGK 2

HS Code 

3504009000

Storage Class

10 - Combustible liquids

ANTI-AKT1 Usage And Synthesis

Uses

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Uses

Anti-AKT1 (Ab-473) antibody has been used in western blotting and immunostaining.

Uses

Anti-AKT antibody produced in rabbit has been used in Western Blotting.

General Description

Anti-AKT Antibody detects endogenous levels of total AKT protein.

Biochem/physiol Actions

AKT serine/threonine kinase 1 (AKT1) acts as a regulator of cell-survival and anti-apoptotic functions. This protein has a crucial role in tumorigenesis. Mutations in the gene are associated with the pathogenesis of prostate cancer (PC). Elevated expression of the gene has been observed in human gastric cancer. Aberrations in AKT1 signaling leads to the development of schizophrenia.

Source

Rabbit

Reactivity

Human;Mouse;Rat;Monkey;Guinea Pig

Background

The p21-activated kinase family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth. Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK. Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1. Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1, and Ser192 and Ser197 of PAK2. Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation. Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 affects kinase activity. Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck. PAK4, PAK5/7, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region. Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4. PAK family members are widely expressed, and often overexpressed in human cancer.

References

[1] Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-62.
[2] Daniels, R.H. et al. (1998) EMBO J. 17, 754-64.
[3] King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-9.
[4] Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-43.
[5] Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8.
[6] Lei, M. et al. (2000) Cell 102, 387-97.
[7] Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-53.
[8] Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-17.
[9] Abo, A. et al. (1998) EMBO J. 17, 6527-640.
[10] Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-33.

ANTI-AKT1(2210301-92-9)Related Product Information

• ATP1A1 antibody
• Pou3f1 Antibody
• SOX13 antibody
• Anti-FBLN2 Antibody
• CCDC88A antibody
• LHCGR antibody
• CRHR1 antibody
• SLC20A1 ANTIBODY
• ROS1 antibody
• CTR9 antibody