4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO-
4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO- Basic information
- Product Name:
- 4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO-
- Synonyms:
-
- Calceintetraethylacid, >97%
- 4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO-
- 3H-IndoliuM, 2-[3-[1,3-dihydro-3,3-diMethyl-1-[6-oxo-6-(2-propyn-1-ylaMino)hexyl]-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-diMethyl-5-sulfo-coMpd. with N,N-diethylethanaMine (1:2)
- Calcein tetraethyl acid
- Glycine, N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-4',5'-diyl)bis(methylene)]bis[N-(carboxymethyl)-
- 2,2',2'',2'''-(((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetic acid
- Fluorescein-4',5'-bis(methyliminodiaceticacid)
- Calcein (free acid), green fluorescent Ca2+ indicator
- CAS:
- 207124-64-9
- MF:
- C30H26N2O13
- MW:
- 622.53
- Product Categories:
-
- marker
- Mol File:
- 207124-64-9.mol
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4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO- Chemical Properties
- solubility
- DMSO (Slightly, Heated), Methanol (Slightly, Heated, Sonicated)
- form
- Solid
- color
- Yellow to Dark Yellow
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4' 5'-BIS(N N-BIS(CARBOXYMETHYL)AMINO- Usage And Synthesis
Uses
Fluorescein Bis(methylene)]bis[N-(carboxymethyl)glycine] is an intermediate used in the synthesis of Calcein AM (C125400), which is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.
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