ANTI-NEOMYCIN PHOSPHOTRANSFERASE II
ANTI-NEOMYCIN PHOSPHOTRANSFERASE II Basic information
- Product Name:
- ANTI-NEOMYCIN PHOSPHOTRANSFERASE II
- Synonyms:
-
- ANTI-NEOMYCIN PHOSPHOTRANSFERASE II
- ANTI-NPT II
- ANTI-NPTII (C-TERMINAL)
- Anti-NPTII (C-terminal) antibody produced in rabbit
- Anti-NPTII antibody produced in rabbit
- MW:
- 0
- Mol File:
- Mol File
ANTI-NEOMYCIN PHOSPHOTRANSFERASE II Chemical Properties
- storage temp.
- -20°C
- form
- buffered aqueous solution
MSDS
- Language:English Provider:SigmaAldrich
ANTI-NEOMYCIN PHOSPHOTRANSFERASE II Usage And Synthesis
Uses
Anti-NPTII antibody has been used in immunoblotting.
General Description
Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.
Biochem/physiol Actions
The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.