NPGB Chemical Properties

Melting point:

241-243 °C

storage temp. 

-20°C

form 

Solid

color 

White to off-white

BRN 

8024554

Safety Information

Hazard Codes 

Xi

Risk Statements 

36

Safety Statements 

26

WGK Germany 

3

F 

8-10

MSDS

• Language:English Provider:SigmaAldrich

NPGB Usage And Synthesis

Uses

4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:

• as a substrate for trypsin for active site titration experiments
• for pre-treating of mosquito eggs in the interplasmid transposition assay
• as a component of isotonic buffer to moisten filter paper for mosquito embryo collection

General Description

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.

Biochem/physiol Actions

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.

References

[1] BHUMI PATEL . L. donovani XPRT: Molecular characterization and evaluation of inhibitors[J]. Biochimica et biophysica acta. Proteins and proteomics, 2018, 1866 3: Pages 426-441. DOI:10.1016/j.bbapap.2017.12.002.
[2] FRANK S. STEVEN Margaret M G. Studies on the Molecular Mechanism of Mersalyl and 4-Aminophenylmercuric Acetate Re-activation of Trypsin-Thiol Complexes[J]. The FEBS journal, 1980, 109 2: 567-573. DOI:10.1111/j.1432-1033.1980.tb04829.x.
[3] YING-YING HE . Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom[J]. Peptides, 2008, 29 10: Pages 1692-1699. DOI:10.1016/j.peptides.2008.05.025.
[4] J.A. SCHIFFERLI G. S. A simple two-step procedure for the preparation of the first component of human complement (C1) in its native form[J]. Journal of immunological methods, 1985, 76 2: Pages 283-288. DOI:10.1016/0022-1759(85)90305-9.
[5] J. M. KAMINSKI. Synthesis and inhibition of human acrosin and trypsin and acute toxicity of aryl 4-guanidinobenzoates[J]. Journal of Medicinal Chemistry, 1986, 29 4: 514-519. DOI:10.1021/jm00154a015.

NPGB(19135-17-2)Related Product Information

• 1-phenylguanidine
• 4-(Methylamino)benzoic acid
• 4-AMINOBENZOIC ACID HCL
• 4-BENZYLOXYANILINE
• 4-Guanidinobenzoic acid hydrochloride
• METHYL 4-METHYLAMINOBENZOATE
• 1-BENZYLOXY-4-NITROBENZENE
• 4-NITROPHENYL BENZOATE
• 4-Benzyloxyaniline hydrochloride
• Phenyl benzoate
• PHENYL 4-AMINOBENZOATE
• N-P-TOLYL-GUANIDINE HYDROCHLORIDE
• P-PHENETIDINE HYDROCHLORIDE
• N-P-TOLYL-GUANIDINE
• NPGB
• METHYL 4-AMINOBENZOATE HYDROCHLORIDE
• 4-(PHENOXYMETHYL)ANILINE
• 4-nitrophenyl 4'-guanidinobenzoate