2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein Chemical Properties

storage temp. 

-20°C

solubility 

DMF: 5 mg/ml; DMSO: 15 mg/ml; DMSO:PBS (pH 7.2) (1:2): 0.25 mg/ml; Ethanol: 5 mg/ml

form 

A crystalline solid

color 

Yellow to orange

Appearance

Solid Powder

BRN 

8375770

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22-36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

F 

8

MSDS

• Language:English Provider:SigmaAldrich

2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein Usage And Synthesis

Description

BCECF is a fluorescent cytoplasmic pH-sensitive indicator. pH is measured by the ratio of emission intensity.

Uses

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein has been used in a fluorescence-based screening assay, to determine the pH.

Uses

2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein is useful for pH measurements in intercellular spaces of epithelial cell monolayers, interstitial spaces of normal and neoplastic tissue and isolated cell fractions. Reliable measure of in vitro cellular cytotoxicity.

Biochem/physiol Actions

Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.

Excitation / Emission Maximum

440-490 nm/535 nm

2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein(85138-49-4)Related Product Information

• 1-(2-METHOXYPHENYL)ETHANOL
• 4-Methylbenzhydrol
• 5-METHYLISOPHTHALIC ACID
• 2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
• 3-FORMYL-BENZOIC ACID ETHYL ESTER
• 2,5-Dimethylbenzoic acid
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• 4-Ethylbenzoic acid
• 3-PHENOXYPHENOL
• BCECF-AM
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• 3-(4-METHOXYPHENYL)-1-PROPANOL
• 5-Carboxyfluorescein
• 4-BENZYL-BENZALDEHYDE
• o-Cresolphthalein
• 4-ISOPROPYLBENZYL ALCOHOL
• 5(6)-Carboxyfluorescein