2,2',5,5'-Tetrachlorobenzidine Chemical Properties

Melting point:

135-140 °C(lit.)

Boiling point:

477.67°C (rough estimate)

Density 

1.5309 (rough estimate)

refractive index 

1.6000 (estimate)

storage temp. 

-70°C

solubility 

soluble in Methanol

form 

powder to crystal

pka

1.58±0.13(Predicted)

color 

Light yellow to Yellow to Orange

biological source

human

Water Solubility 

water: soluble

CAS DataBase Reference

15721-02-5(CAS DataBase Reference)

IARC

3 (Vol. 27, Sup 7) 1987

EPA Substance Registry System

[1,1'-Biphenyl]-4,4'-diamine, 2,2',5,5'-tetrachloro- (15721-02-5)

Safety Information

Hazard Codes 

T

Risk Statements 

61

Safety Statements 

53-45

WGK Germany 

3

RTECS 

DD1751000

HS Code 

2921.59.8090

MSDS

• Language:English Provider:SigmaAldrich

2,2',5,5'-Tetrachlorobenzidine Usage And Synthesis

Uses

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
• oxidative stress experiments on PKM2.
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).

Definition

ChEBI: 2,2',5,5'-Tetrachlorobenzidine is a member of biphenyls and a dichlorobenzene.

Production Methods

2,2' ,5,5' -Tetrachlorobenzidine is made by the reduction of 2,5-dichloronitrobenzene with zinc dust and sodium hydroxide solution. The subsequent rearrangement is carried out using sulfuric acid or hydrochloric acid. The dihydrochloride melts at 230 ℃. Tetrachlorobenzidine is marketed as the free base and is used to produce yellow pigments.

Biological Activity

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.

Safety Profile

Suspected carcinogen with experimental carcinogenic and tumorigenic data. When heated to decomposition it emits very toxic fumes of NO, and Cl-.

2,2',5,5'-Tetrachlorobenzidine(15721-02-5)Related Product Information

• HYDRAZINE
• Biphenyl
• 3,3'-Dichlorobenzidine
• Sodium diphenylamine-4-sulfonate
• 4-Aminodiphenylamine
• Diphenylamine
• 2,2',5-TRICHLOROBIPHENYL
• 2,5-DICHLOROTOLUENE
• 3,3'-Dichlorobenzidine dihydrochloride
• 2,3',5-TRICHLOROBIPHENYL
• Benzidine dihydrochloride
• Pigment Yellow 81
• 2,5-DICHLOROBIPHENYL
• 2,2'-dichlorobenzidine
• 2-CHLOROBIPHENYL
• 2,2'-DICHLOROBIPHENYL
• 3-CHLOROBIPHENYL
• 2,2',5,5'-TETRACHLOROBIPHENYL