bisBenziMide H 33342 trihydrochloride Chemical Properties

storage temp. 

Inert atmosphere,Store in freezer, under -20°C

solubility 

Soluble in water, N,N-dimethylformamide, methano

pka

11.08 ± 0.69, most acidic, temperature: 25 °C; 7.66 ± 0.42, most basic(at 25℃)

form 

A crystalline solid

color 

Yellow to green powder or solid

PH

1.7 (20°C)

Appearance

Solid Powder

Water Solubility 

H2O: 20mg/mL
phosphate buffer: precipitates

λmax

350 nm (H2O/DNA); 357 nm (MeOH/H+)

Biological Applications

Detecting β-amyloid (Aβ) plaques; detecting/measuring microorganisms; identifying/counting cells; studying multidrug resistance; apoptosis assay; cell cycle assay; cell proliferation assay; measuring membrane potential; treating disc degenerative disease, erectile dysfunction

Major Application

Photoresists

InChIKey

OTMWRDPOAAYNCR-UHFFFAOYSA-N

SMILES

CN1CCN(C2=CC=C3N=C(C4=CC=C5N=C(C6C=CC(OCC)=CC=6)NC5=C4)NC3=C2)CC1.Cl

Safety Information

WGK Germany 

WGK 3

Storage Class

11 - Combustible Solids

bisBenziMide H 33342 trihydrochloride Usage And Synthesis

Description

Hoechst 33342 is a cell-permeable DNA dye that binds to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA.

Uses

Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells. It has been used to detect BrdU incorporation into cells and in studying the initial stages apoptosis and cellcycle distribution., Chromosomes th at are dividing or replicating will not stain with this dye. ', 'Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Biological Activity

Membrane-permeable, fluorescent DNA stains with low cytotoxicity th at intercalate in A-T regions of DNA.

storage

-20°C

Excitation / Emission Maximum

350 nm/461 nm

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