NSC 95397 Chemical Properties

storage temp. 

-20°C

solubility 

DMSO: 16 mg/mL

form 

Orange solid

color 

orange, solid

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.

Safety Information

Hazard Codes 

Xi

WGK Germany 

3

HazardClass 

IRRITANT

MSDS

• Language:English Provider:SigmaAldrich

NSC 95397 Usage And Synthesis

Description

NSC 95397 (93718-83-3) is a potent and selective inhibitor of Cdc25 phosphatase (Ki? = 32, 96 and 40 nM for Cdc25A, B and C respectively). NSC-95397 inhibits the growth of several human tumor cell lines and blocks G2/M cell cycle transition.1 Induces cell cycle arrest, phosphorylation of EGFR and activation of ERK-1 and -2.2 Inhibits carboxyl-terminal binding protein a transcriptional corepressor that suppresses multiple pro-apoptotic and epithelial genes.3 Induces eryptosis in human erythrocytes.4

Uses

NSC 95397 is a quinone-based Cdc25 inhibitor, caspase-3 activator, ERK activator, and MKP inhibitor.

Definition

ChEBI: 2,3-bis(2-hydroxyethylthio)naphthalene-1,4-dione is a member of 1,4-naphthoquinones.

Biological Activity

Potent and selective irreversible inhibitor of Cdc25 dual specificity phosphatases (K i values are 32, 96 and 40 nM for inhibition of Cdc25A, -B and -C respectively). Displays 125-180-fold selectivity over VH1-related dual-specificity phosphatase and protein tyrosine phosphatase 1b. Inhibits carcinoma cell growth and blocks G 2 /M phase transition in vitro .

Biochem/physiol Actions

Irreversible Cdc25 dual specificity phosphatase inhibitor.

storage

Desiccate at -20°C

References

[1] J. LAZO. Identification of a potent and selective pharmacophore for Cdc25 dual specificity phosphatase inhibitors.[J]. Molecular Pharmacology, 2002, 61 4 1: 720-728. DOI:10.1124/mol.61.4.720
[2] IRA MELCHHEIER. Quinone-induced Cdc25A inhibition causes ERK-dependent connexin phosphorylation[J]. Biochemical and biophysical research communications, 2005, 327 4: Pages 1016-1023. DOI:10.1016/j.bbrc.2004.12.107
[3] MELANIE A. BLEVINS . Small Molecule, NSC95397, Inhibits the CtBP1-Protein Partner Interaction and CtBP1-Mediated Transcriptional Repression[J]. SLAS Discovery, 2015, 20 5: Pages 663-672. DOI:10.1177/1087057114561400
[4] MOHAMED JEMAÀ. Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397[J]. Cellular Physiology and Biochemistry, 2016, 40 1: 597-607. DOI:10.1159/000452573

NSC 95397(93718-83-3)Related Product Information

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