NSC 95397 Chemical Properties

storage temp. 

-20°C

solubility 

DMSO: 16 mg/mL

form 

Orange solid

color 

orange, solid

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.

Safety Information

Hazard Codes 

Xi

WGK Germany 

3

HazardClass 

IRRITANT

MSDS

• Language:English Provider:SigmaAldrich

NSC 95397 Usage And Synthesis

Description

NSC 95397 (93718-83-3) is a potent and selective inhibitor of Cdc25 phosphatase (Ki? = 32, 96 and 40 nM for Cdc25A, B and C respectively). NSC-95397 inhibits the growth of several human tumor cell lines and blocks G2/M cell cycle transition.1 Induces cell cycle arrest, phosphorylation of EGFR and activation of ERK-1 and -2.2 Inhibits carboxyl-terminal binding protein a transcriptional corepressor that suppresses multiple pro-apoptotic and epithelial genes.3 Induces eryptosis in human erythrocytes.4

Uses

NSC 95397 is a quinone-based Cdc25 inhibitor, caspase-3 activator, ERK activator, and MKP inhibitor.

Definition

ChEBI: 2,3-bis(2-hydroxyethylthio)naphthalene-1,4-dione is a member of 1,4-naphthoquinones.

Biological Activity

Potent and selective irreversible inhibitor of Cdc25 dual specificity phosphatases (K i values are 32, 96 and 40 nM for inhibition of Cdc25A, -B and -C respectively). Displays 125-180-fold selectivity over VH1-related dual-specificity phosphatase and protein tyrosine phosphatase 1b. Inhibits carcinoma cell growth and blocks G 2 /M phase transition in vitro .

Biochem/physiol Actions

Irreversible Cdc25 dual specificity phosphatase inhibitor.

storage

Desiccate at -20°C

References

1) Lazo et al. (2002), Identification of a potent and selective pharmacophore for Cdc25 dual specificity phosphatase inhibitors; Mol. Pharmacol., 61 720 2) Melchheier et al. (2005), Quinone-induced Cdc25A inhibition causes ERK-dependent connexin phosphorylation; Biochem. Biophys. Res. Commun., 327 1016 3) Blevins et al. (2015), Small Molecule, NSC95397, inhibits the CtBP1-Protein Partner Interaction and CtBP1-Mediated Transcriptional Repression; J. Biomol. Screen., 20 663 4) Jemaa et al. (2016), Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397; Cell. Physiol. Biochem., 40 597

NSC 95397(93718-83-3)Related Product Information

• ZM 447439
• CYCLOPAMINE
• S-Trityl-L-cysteine
• AG 490
• 2,3-BIS(MERCAPTOACETIC ACID)-1,4-NAPHTHALENEDIONE
• 2,3-BIS(METHYLTHIO)-1,4-NAPHTHALENEDIONE
• NSC 95397