N-Acetylpuromycin Chemical Properties

Density 

1.47±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

Soluble in DMSO (up to 70 mg/ml) or in DMF (up to 55 mg/ml)

form 

solid

pka

12.98±0.70(Predicted)

color 

White

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 2 months.

N-Acetylpuromycin Usage And Synthesis

Description

N-Acetylpuromycin is a non-ribotoxic form of the antibiotic puromycin that is formed in puromycin-resistant S. alboniger that endogenously express puromycin-acetyltransferase. In AD293(Pr) cells that express puromycin-N-acetyltransferase, the enzyme responsible for the biosynthesis of N-acetylpuromycin, puromycin application induces downregulation of the negative regulators of TGF-β signalling SnoN and Ski without activating MAPK or inhibition of protein synthesis.

Uses

N-Acetylpurinomycin downregulates SnoN and Ski protein expression and promotes TGF-β signaling, independently of MAPK activation.

storage

Store at -20°C

References

[1] JACQUELINE HERNÁNDEZ-DAMIÁN . Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals[J]. Biochimica et biophysica acta. General subjects, 2013, 1830 11: Pages 5049-5058. DOI:10.1016/j.bbagen.2013.07.006

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