Proteinase K, recombinant, PCR Grade Chemical Properties

storage temp. 

2-8°C

form 

buffered aqueous solution (18 ± mg/mL; pH 7.5)

Specific Activity

~2units/mg protein

Proteinase K, recombinant, PCR Grade Usage And Synthesis

Uses

• This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
• The enzyme promotes cell lysis by activating a bacterial autolytic factor.
• Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
• The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.
• Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

General Description

Proteinase K is a subtilisin-related serine protease that has no pronounced cleavage specificity.
The enzyme is also available as a lyophilizate.