HtrA2 antibody Usage And Synthesis

Source

Rabbit

Applications

HTRA2 antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Recommended dilution range for Western blot analysis is 1:500, ICC/IF is 1:100.

Reactivity

Human;Mouse;Rat;Monkey

Immunogen

Anti-human HTRA2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HTRA2 protein 134-458 amino acids purified from E. coli.

Background

HtrA2 also called Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.

References

[1] Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
[2] Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
[3] Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
[4] Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
[5] Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
[6] van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
[7] Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
[8] Jones, J.M. et al. (2003) Nature 425, 721-727.
[9] Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
[10] Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.

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