LDHA Antibody Usage And Synthesis

Source

Rabbit

Applications

LDHA antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 8,000.
Recommended starting dilution is

Reactivity

Human;Mouse;Rat;Monkey

Immunogen

Anti-human LDHA mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human LDHA amino acids 1-332 purified from E. coli.

Background

LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

References

[1] Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
[2] Semenza, G.L. (2007) Biochem J 405, 1-9.