TETRAMETHYLRHODAMINE Chemical Properties

storage temp. 

-20°C

solubility 

DMSO, DMF

form 

solid

Appearance

Dark red amorphous solid

InChI

InChI=1S/C24H22N2O3.ClH/c1-25(2)15-9-11-19-21(13-15)29-22-14-16(26(3)4)10-12-20(22)23(19)17-7-5-6-8-18(17)24(27)28;/h5-14H,1-4H3;1H

InChIKey

WGTODYJZXSJIAG-UHFFFAOYSA-N

SMILES

C1(C2C=CC=CC=2C(=O)O)=C2C=C/C(=[N+](/C)\C)/C=C2OC2C=C(N(C)C)C=CC1=2.[Cl-]

Safety Information

WGK Germany 

3

TETRAMETHYLRHODAMINE Usage And Synthesis

Description

The red-fluorescent TAMRA Azide (TAMRA-PEG4-Azide) can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.TAMRA (tetramethylrhodamine) is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

Uses

Can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following an azide-alkyne click chemistry reaction.

Spectral Properties: Abs/Em = 546/565 nm

Definition

ChEBI: Tetramethylrhodamine chloride is an organic chloride salt. It has a role as a fluorochrome. It contains a tetramethylrhodamine.

reaction suitability

reaction type: click chemistry

Abs/Em Maxima

553/575 nm

Extinction Coefficient

92,000

Spectrally Similar Dyes

Alexa Fluor® 546, Atto™ 543, CF® 543 Dye

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