5-Propargylamino-3'-azidomethyl-dUTP Usage And Synthesis

Description

5-Propargylamino-3'-azidomethyl-dUTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS).

Uses

5-Propargylamino-3'-azidomethyl-dUTP is a nucleoside molecule extracted from patent WO2004018497A2, compound 5. 5-Propargylamino-3'-azidomethyl-dUTP can be used in DNA synthesis and DNA sequencing[1]. 5-Propargylamino-3'-azidomethyl-dUTP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

References

[1] Milton J, et, al. Modified nucleotides for polynucleotide sequencing. WO2004018497A2.