O-Propargyl-PuroMycin
O-Propargyl-PuroMycin Basic information
- Product Name:
- O-Propargyl-PuroMycin
- Synonyms:
-
- O-Propargyl-PuroMycin
- Adenosine, 3'-[[(2S)-2-amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyl-
- O-propargyl-puromycin (OPP)
- OPropargylPuromycin,O Propargyl Puromycin
- O-Propargylpuromycin, protein marker for imaging
- O-Propargyl-Puromycin, 10 mM in DMSO
- O-propargyl-puromycine (OPP) (CCT-1407)
- CAS:
- 1416561-90-4
- MF:
- C24H29N7O5
- MW:
- 495.53
- Mol File:
- 1416561-90-4.mol
O-Propargyl-PuroMycin Chemical Properties
- Density
- 1.44±0.1 g/cm3(Predicted)
- storage temp.
- Store at -20°C
- solubility
- DMF: soluble; DMSO: soluble
- form
- A crystalline solid
- pka
- 13.00±0.70(Predicted)
- color
- White to off-white
O-Propargyl-PuroMycin Usage And Synthesis
Uses
O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor. O-Propargyl-Puromycin is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
in vivo
Tissues from uninjected mice shows low nonspecific staining, whereas tissues from O-propargyl-puromycin-injected mice display specific patterns of O-propargyl-puromycin incorporation into nascent proteins. In the small intestine, translation is strongest in cells in the crypts and at the base of intestinal villi, consistent with the high proliferative and secretory activity of these cells. The stain is particularly strong in Paneth cells, which are located close to the base of the crypts and are filled with secretory vesicles. The intense O-propargyl-puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated, O-propargyl-puromycin-conjugated secretory proteins are translocated into the endoplasmic reticulum (ER) lumen[1].
storage
Store at -20°C
References
[1] Liu J, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):413-8. DOI:10.1073/pnas.1111561108
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