HEPTANOYL THIO-PC Chemical Properties

solubility 

DMF: 10 mg/ml
DMSO: 2 mg/ml
Ethanol: 30 mg/ml
Ethanol: PBS (pH 7.2)(1:8): .5 mg/ml

HEPTANOYL THIO-PC Usage And Synthesis

Description

Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-PC is a commonly-used colorimetric substrate for all PLA₂s, with the exception of cPLA₂ and platelet-activating factor acetylhydrolase (PAF-AH). Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-PC is a commonly-used colorimetric substrate for all PLA₂s, with the exception of cPLA₂ and platelet-activating factor acetylhydrolase (PAF-AH). Heptanoyl thio-PC is an analog of diheptanoyl thio-PC that contains an ether-linked saturated C₁₆ moiety at the sn-1 position rather than a heptanoyl thiol ester. Porcine pancreatic and bee venom sPLA₂ enzymes exhibit 10-13 fold less activity when assayed with heptanoyl thio-PC compared to diheptanoyl thio-PC. This decrease in activity has not been thoroughly investigated.

References

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