Saccharin 1-methylimidazole (SMI) Chemical Properties

storage temp. 

Store at -20°C

solubility 

DMSO:52.67(Max Conc. mg/mL);198.54(Max Conc. mM)
Water:53.0(Max Conc. mg/mL);199.78(Max Conc. mM)
Ethanol:31.5(Max Conc. mg/mL);118.74(Max Conc. mM)
DMF:5.0(Max Conc. mg/mL);18.85(Max Conc. mM)
PBS (pH 7.2):10.0(Max Conc. mg/mL);37.69(Max Conc. mM)

form 

A solid

color 

Light yellow to orange

Saccharin 1-methylimidazole (SMI) Usage And Synthesis

Description

Saccharin 1-methylimidazole is a chemical activator that has been commonly used in solid-phase synthesis of DNA and RNA oligonucleotides. It catalyzes the phosphoramidite coupling reaction of the 5'' hydroxy of immobilized nucleosides and the amine group of nucleoside phosphoramidite. Saccharin 1-methylimidazole has been used to synthesize single-stranded DNA (ssDNA) oligonucleotides that are resistant to nuclease digestion.

Uses

Saccharin 1-Methylimidazole is a reagent used for nucleic acid synthesis (1,2).

References

[1] RUSSELL M A, LAWS A P, ATHERTON J H, et al. The mechanism of the phosphoramidite synthesis of polynucleotides[J]. Organic & Biomolecular Chemistry, 2008, 18: 3270-3275. DOI: 10.1039/b808999j
[2] De Souza, F.I., Zumiotti, A.V., and Da Silva, C.F. Neuregulins 1-α and 1-β on the regeneration the peripheral nerves[J]. Acta Ortop Bras.