Fluo-3FF (potassium salt) Chemical Properties

storage temp. 

Store at -20°C

solubility 

Water: soluble

form 

A solid

Fluo-3FF (potassium salt) Usage And Synthesis

Description

Fluo-3FF is a fluorescent calcium indicator. It is a di-fluorinated analog of Fluo-3 (Item Nos. 20402 | 20403 | 20404) that displays about a 100-fold lower affinity than Fluo-3 for calcium (K_ds = 42 and 0.4 μM, respectively). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Fluo-3FF has excitation/emission maxima of approximately 506 and 526 nm, respectively.

Uses

Fluo-3FF pentapotassium is a cell-permeable acetoxy-methyl ester of Fluo-3FF, a fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 M, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

References

[1] GAVRIEL DAVID  Ellen F B  John N Barrett. Stimulation-induced changes in [ca2+] in lizard motor nerve terminals[J]. Journal of Physiology-London, 2004, 504 1: 83-96. DOI: 10.1111/j.1469-7793.1997.083bf.x