Ac-DNLD-AMC Chemical Properties

Boiling point:

1211.3±65.0 °C(Predicted)

Density 

1.402±0.06 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

Formic Acid: 1 mg/ml

form 

A lyophilized powder

pka

4.09±0.10(Predicted)

Ac-DNLD-AMC Usage And Synthesis

Description

Ac-DNLD-AMC is a fluorogenic caspase-3 substrate. Upon enzymatic cleavage by caspase-3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-3 activity. AMC displays excitation/emission maxima of 340-360 and 440-460 nm, respectively.

Uses

Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity[1][2].

References

[1] Kulikov AV, et al. Targeting mitochondria by α-tocopheryl succinate overcomes hypoxia-mediated tumor cell resistance to treatment. Cell Mol Life Sci. 2014 Jun;71(12):2325-33. DOI:10.1007/s00018-013-1489-8
[2] Yoshida K, et al. Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells. Cell Immunol. 2009;255(1-2):61-8. DOI:10.1016/j.cellimm.2008.10.006