PHOSPHATE BUFFERED SALINE Chemical Properties

Density 

1.19g/cm3 at 20—25°C
1.22g/cm3 at 20—25°C

storage temp. 

2-8°C

solubility 

H₂O: 1 tablet/200 mL, clear, colorless

form 

tablets

color 

white

PH

7.4 ( in solution)

Water Solubility 

water: soluble

λmax

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

Specific Activity

(Microbial DNA free buffer)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-24/25-22

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

PHOSPHATE BUFFERED SALINE Usage And Synthesis

Uses

Phosphate buffered saline has been used in sample preparation for quantifying the wound fluid proteins from pressure ulcers in patients with spinal cord injury (SCI). It has also been used for cell rinsing in various biological researches.

Uses

• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.

Uses

Phosphate buffered saline has been used-

• for the production of platinum nanoparticles (NP-Pt) hydrocolloids
• for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
• for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
• for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
• for suspension of cell pellets during centrifugation
• in immunoassay procedures

General Description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Biological Activity

Dulbeccoμs Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

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