PKM2 antibody Usage And Synthesis

Source

Rabbit

Applications

PKM2 antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.  

Reactivity

Human;Mouse;Rat;Monkey

Immunogen

Anti-human PKM2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PKM2 amino acids 1-531 purified from E. coli.

Background

Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.

References

[1] Christofk, H.R. et al. (2008) Nature 452, 230-3.
[2] Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
[3] Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
[4] Israelsen, W.J. et al. (2013) Cell 155, 397-409.