MMP-13 SUBSTRATE, FLUOROGENIC Chemical Properties

storage temp. 

-20°C

solubility 

DMSO: 5mg/mL

form 

lyophilized solid

color 

yellow

MMP-13 SUBSTRATE, FLUOROGENIC Usage And Synthesis

Description

Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 is a fluorogenic substrate for matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-26. 7-methoxycoumarin-4-acetyl (Mca) is released by cleavage of Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2, of which the fluorescence can be used to quantify MMP-1, MMP-3 and MMP-26 activity.

Uses

Matrix metalloproteinases (MMP) are a family of zinc-dependent endopeptidases th at degrade matrix proteins. MMPs include collagenases, gelatinases, matrilysins, enamelysins, metalloelastases, stromelysins and other structural protein and receptor lysins. The activity of these enzymes is selectively inhibited or measured by appropriately labeled peptides. MCA-Pro-Cha-Gly-Nva-His-Ala-DNP-DPA-NH2 is used as a fluorogenic substrate for matrix metalloprotein-13 (collagenase-3, MMP-13).

Biological Activity

Primary Target
A quenched fluorescence substrate for MMP-13 (collagenase-3)', 'kcat/Km = 1.09 x 106 M-¹ sec-¹ for MMP-13 (collagenase-3)

Sequene

Mca-Pro-Cha-Gly-Nva-His-Ala-Dap(Dnp)-NH2