6,8-DIFLUORO-4-METHYL-UMBELLIFERYL PHOSPHATE Chemical Properties

Boiling point:

496.0±55.0 °C(Predicted)

Density 

1.716±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

29.21mg/ml in DMSO

form 

A solid

pka

1.48±0.10(Predicted)

color 

White to off-white

Appearance

Solid Powder

Safety Information

Safety Statements 

22-24/25

6,8-DIFLUORO-4-METHYL-UMBELLIFERYL PHOSPHATE Usage And Synthesis

Description

6,8-difluoro-4-methylumbelliferyl phosphate is a fluorogenic substrate for protein tyrosine phosphatase.

Uses

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

Definition

ChEBI: 6,8-difluoro-4-methylumbelliferyl phosphate is a member of coumarins. It is functionally related to a coumarin. It is a conjugate acid of a 6,8-difluoro-4-methylumbelliferyl phosphate (2-).

Biological Activity

DiFMUP (6,8-difluoro-4-methylumbelliferyl phosphate), a fluorinated analog of MUP, is a fluorescent synthetic substrate for continuous assessment of phosphatase activity. DiFMUP excitation/emission maxima are 358/450 nm.

storage

Store at -20°C

References

[1] Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8. DOI:10.1006/abio.1999.4202
[2] Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8. DOI:10.1016/j.ab.2004.11.047

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