Solvent Green 7 Chemical Properties

Melting point:

62-63.5 °C(lit.)

Boiling point:

171-178 °C10 mm Hg(lit.)

Density 

2.15

vapor pressure 

0Pa at 25℃

Flash point:

250 °C

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

methanol: 0.1 g/mL, clear

Colour Index 

59040

form 

Powder

pka

7.3, (at 22℃)

color 

yellow

Water Solubility 

300 g/L (25 ºC)

λmax

403 nm (Buffer pH 4.0); 454 nm (Buffer pH 9.0)

Merck 

7954

BRN 

4107272

Major Application

Adhesives; agrochemicals; ammonia sensor; carbon dioxide sensor; oxygen sensor; anionic surfactants sensor; measuring the size of the organoclay aggregates; detecting cracks/flaws in metallic surfaces, refrigerant leaking; detergents; correction fluid; highlighters; inks; markers; paints; pencils; toners; chemically amplified resist; color filters; chemiluminescent materials; electrophoretic display (EPD) film materials; electroluminescent displays/materials; fuel cell; lasers; liquid crystal displays; luminescent materials; nanoparticles; papers; photoresist processes; photonic ionic liquids; soils; solar cell; sol–gel materials/processes/thin films; sonophotoluminescence; water tracers; evaluating liquid water flow in wood during drying

Biological Applications

Antiperspirants; bath preparations; cleansing products; cosmetics; contact lens; hair dyes; hygiene products/soaps; food products; controlled-release system for proteins; drug delivery; in ophthalmology/retinal surgery; sugar sensors (glucose, fructose, lactose, sucrose); as a substrate for measuring acetylcholinesterase (AChE) activity, carbonic anhydrase activity, esterases activity, β-glucuronidase activity, lipase activity, acidic/alkaline phosphatases activity, pyrophosphatase activity, sucrose phosphorylase (SPO) activity, phosphoglucomutase (PGM) activity; treating cancer, infection, immune/autoimmune disease, mastitis, tonsillitis, tuberculosis

LogP

-3.97 at 25℃

CAS DataBase Reference

6358-69-6(CAS DataBase Reference)

EPA Substance Registry System

C.I. Solvent Green 7 (6358-69-6)

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

36/37/38

Safety Statements 

36/37-37/39-26-36

WGK Germany 

3

RTECS 

UR2700000

TSCA 

Yes

HS Code 

29089990

MSDS

• Language:English Provider:Solvent Green 7
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Solvent Green 7 Usage And Synthesis

Chemical Properties

yellow to green-yellow powder

Uses

Pyranine is used as a fluorescent pH indicator with emission change in the physiological pH range. It acts as a dye in optical sensor applications. It finds application as a coloring agent and biological stain. It is also used in the measurement of pH of intracellular fluid in the cells.

Definition

ChEBI: Pyranine is an organic sodium salt. It has a role as a fluorochrome. It contains a pyranine(3-). It derives from a hydride of a pyrene.

Preparation

commonly known as Pyranine. Pyrene-1,3,6,8-tetrasulfonic acid?and 15 ~ 20% sodium hydroxide reflux, also can use ammonia water on hydrolysis reaction, this condition the product contains APTS(8-Aminopyrene-1,3,6-trisulfonic acid?).

Flammability and Explosibility

Not classified

Biological Activity

Pyranine (HPTS; Solvent Green 7) is a pH-sensitive fluorescent indicator. Pyranine is a class of fluorescent chemosensors for Cu+ ions (λex=450 nm, λem=510 nm).

in vitro

Pyranine is capable of discriminating ranges of cations from the Cu + ion, even in competing environment. Pyranine displays a rapid fluorescence response (t 1/2 =1.66 min) towards the Cu + ion, and the micromolar detection limit enables the detection of the ion in environmental samples. The observed stoichiometry of complexation between Pyranine and Cu + is 2:1.The pH-sensitive fluorescent indicator Pyranine is studied to determine its usefulness as probes for the living cornea and anterior chamber. Adequate concentrations are reached in the cornea and anterior chamber after topical administration; measurements can be made by fluorophotometry for many hours. The pH is calculated by measuring the ratio of fluorescent intensity at two excitation wavelengths, I463/I404, a measurement which is dependent on pH but independent of the concentration of the fluorophore and other variables which can alter the intensity. In the rabbit eye, Pyranine in the cornea and anterior chamber is observed to undergo easily measurable changes in fluorescent ratios associated with lid closure and contact lens wear, indicating its sensitivity to mild changes in pH.

Properties and Applications

yellow green. Slightly soluble in glacial acetic acid, 30% hydrochloric acid, insoluble in acetic acid and 10% hydrochloric acid. Mainly used in medicine and cosmetics coloring.

Purification Methods

Purify the salt by chromatography with an alumina column, and elute with n-propanol/water (3:1, v/v). Recrystallise it from aqueous acetone (5:95, v/v) using decolorising charcoal. [Beilstein 1 III 565.] IRRITANT.

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