OPD EASY-tablets Chemical Properties

Melting point:

258 °C (dec.)(lit.)

storage temp. 

2-8°C

solubility 

H₂O: 1 tablet/10 mL, clear, colorless

form 

tablet

color 

white to off-white

PH

1.5

Water Solubility 

Slightly soluble in water; freely soluble in alcohol, chloroform or ether

BRN 

3912045

Stability:

Stable. Incompatible with strong oxidizing agents.

IARC

2B (Vol. 123) 2020

EPA Substance Registry System

1,2-Phenylenediamine dihydrochloride (615-28-1)

Safety Information

Hazard Codes 

Xn,N,T

Risk Statements 

22-40-43-51/53-68-50/53-36-25-20/21-36/37/38

Safety Statements 

36/37-61-60-45-28-26

RIDADR 

UN 2811 6.1/PG 3

WGK Germany 

3

RTECS 

ST0175000

F 

8-10-23

TSCA 

Yes

HazardClass 

6.1(b)

PackingGroup 

III

HS Code 

29215190

Hazardous Substances Data

615-28-1(Hazardous Substances Data)

MSDS

• Language:English Provider:SigmaAldrich

OPD EASY-tablets Usage And Synthesis

Chemical Properties

white to light pink powder

Uses

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H₂SO₄ and read at 492 nm.

Uses

o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).

General Description

Brownish-yellow crystals. Slightly soluble in water. Used in the manufacture of dye.

Air & Water Reactions

Slightly soluble in water.

Reactivity Profile

Acidic organic/inorganic salts, such as OPD EASY-tablets, are generally soluble in water. The resulting solutions contain moderate to high concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.

Purification Methods

Crystallise the salt from dilute HCl (60mL conc HCl, 40mL water, with 2g stannous chloride), after treatment of the hot solution with charcoal by adding an equal volume of conc HCl and cooling in an ice-salt mixture. The crystals are washed with a small amount of conc HCl and dried in a vacuum desiccator over NaOH. [Beilstein 13 IV 38.]

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