3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Chemical Properties

solubility 

Soluble in water, DMSO, DMF, etc.

Appearance

Dark blue solid

ex/em

651/672 nm

ε(extinction coefficient)

261,000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.33

InChIKey

PIQXJQCAOCUQOD-UHFFFAOYSA-N

SMILES

C(C1(C(=[N+](CCCS([O-])(=O)=O)C2=CC=C(S(O)(=O)=O)C=C12)C=CC=CC=C1C(C2=CC(S(O)(=O)=O)=CC=C2N1CCCS(O)(=O)=O)(C)C)C)CCCCC(=O)ON1C(CCC1=O)=O

3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Usage And Synthesis

Description

BP Fluor 647 NHS ester (Alexa Fluor?? 647 NHS Ester equivalent) is an amine reactive bright green-fluorescent dye, it can be labeled to biomolecules directly such as primary antibody, secondary antibody, peptide, etc. The conjugates can be used as a probe for flow cytometry, immunocytochemical assays, including In-protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development. Alexa Fluor?? is registered trademarks of Thermo Fisher Scientific.

Chemical Properties

Appearance: Dark blue solid
ex/em: 651/672 nm
Extinction coefficient (ε): 261,000 L??mol??1??cm??1
Quantum yield (Φ): 0.33

Uses

AF647-NHS ester is an analog of Alexa Fluor 647 (AF647). AF647 is a far-red fluorescent dye with an excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). Storage: Protect from light[1][2].

References

[1] Fernandez A, et al. In vivo characterization of the biodistribution profile of amphipol A8-35. J Membr Biol. 2014;247(9-10):1043-1051. DOI:10.1007/s00232-014-9682-8
[2] Karlsson J K G, Laude A, Hall M J, et al. Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy[J]. Chemistry–A European Journal, 2019, 25(65): 14983-14998.

3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt(407627-60-5)Related Product Information

• 3H-Indolium, 3-(5-carboxypentyl)-2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt, compd. with N,N-diethylethanamine (1:4)
• 3H-Indolium, 3-(5-carboxypentyl)-2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt
• CY5 NHS ESTER
• Sulfo-Cy5 NHS Methyl type | Lumiprobe type
• 3H-Indolium, 3-(5-carboxypentyl)-2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadienyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt, compd. with N,N-diethylethanamine (1:3) (9CI)