CY5 NHS ESTER Chemical Properties

storage temp. 

Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

solubility 

Methanol (Slightly), Water (Slightly)

form 

Solid

color 

Dark Purple to Very Dark Purple

Appearance

Dark blue solid

ex/em

649/672 nm (PBS buffer)

ε(extinction coefficient)

271000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.28

Stability:

Moisture Sensitive

InChIKey

WXWLHDCCGVWTDZ-UHFFFAOYSA-N

SMILES

C(N1C(C(C)(C)C2=CC(S(O)(=O)=O)=CC=C12)=CC=CC=CC1C(C2=CC(S([O-])(=O)=O)=CC=C2[N+]=1CC)(C)C)CCCCC(=O)ON1C(CCC1=O)=O

Safety Information

HS Code 

29339900

CY5 NHS ESTER Usage And Synthesis

Description

During the last years, Cyanine5 (an analog of Cy5^®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5^® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5® and DyLight 649.

Chemical Properties

Appearance: Dark blue solid
ex/em: 649/672 nm (PBS buffer)
Extinction coefficient (ε): 271000 L??mol??1??cm??1
Quantum yield (Φ): 0.28

Uses

Cy5-NHS ester can be used toward targeted ultrasound-assisted cancer-selective chemical labeling and cancer imaging. It can also be used for multipotent protein activation.

Chemical Reactivity

Primary amine

storage

Store at -20°C

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.2

Extinction Coefficient

250000

CF260

0.03

CF280

0.04

Spectrally Similar Dyes

Alexa Fluor® 647, DyLight® 649,

CY5 NHS ESTER(146368-14-1)Related Product Information

• EC 3.1.1.1
• Cy3 NHS Ester
• Cy7
• Cy3
• Calcein tetraethyl ester
• CY3 NHS ESTER
• Cy5
• 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt, potassium salt (1:3)
• Cyanine5 NHS ester
• 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt, compd. with N,N-diethylethanamine (1:3)