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Fluorexon

Product Name
Fluorexon
CAS No.
1461-15-0
Chemical Name
Fluorexon
Synonyms
CALCEIN;CALCEINE;FLUOREXON;FLUREXONE;Nsc 298193;FLUOREXONE;Oftasceine;oftasccine;CALCEIN(RG);CALCEIN, INDICATOR
CBNumber
CB0146553
Molecular Formula
C30H26N2O13
Formula Weight
622.54
MOL File
1461-15-0.mol
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Fluorexon Property

Melting point:
200 °C
Boiling point:
662.7°C (rough estimate)
Density 
1.4388 (rough estimate)
refractive index 
1.5800 (estimate)
storage temp. 
Store at +5°C to +30°C.
solubility 
H2O: soluble
form 
Powder
pka
6.67(at 25℃)
color 
Yellow-orange to orange-brown
PH Range
Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
Water Solubility 
insoluble
λmax
494nm
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Calcium indicator; fluoride indicator; iron indicator; mercury indicator; detecting nucleic acids,proteins; treating osteoporosis; drug delivery systems
Major Application
Chemical-mechanical polishing, stabilizing liposomes, cell motility assay, detecting nucleic acids, virus, screening safety of gastric mucosa, MDR inhibitors, delivery, imaging atherosclerotic plaque
CAS DataBase Reference
1461-15-0(CAS DataBase Reference)
EPA Substance Registry System
Glycine, N,N'-[(3',6'- dihydroxy-3-oxospiro[isobenzofuran- 1(3H),9'-[9H]xanthene]- 2',7'-diyl)bis( methylene)]bis[N-(carboxymethyl)- (1461-15-0)
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Safety

Hazard Codes 
Xn
Risk Statements 
20/21/22-36/37/38
Safety Statements 
22-24/25-36/37/39-26
WGK Germany 
3
10
HS Code 
32041900
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
1.02315
Product name
Calcein indicator
Purity
for metal determination
Packaging
1023150005
Price
$169
Updated
2018/11/23
Cayman Chemical
Product number
16221
Product name
Calcein
Packaging
1g
Price
$25
Updated
2018/11/13
Cayman Chemical
Product number
16221
Product name
Calcein
Packaging
5g
Price
$44
Updated
2018/11/13
Cayman Chemical
Product number
16221
Product name
Calcein
Packaging
10g
Price
$75
Updated
2018/11/13
Cayman Chemical
Product number
16221
Product name
Calcein
Packaging
25g
Price
$138
Updated
2018/11/13
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Fluorexon Chemical Properties,Usage,Production

Chemical Properties

orange crystals

Uses

Calcein is used for determination of Ca in biological systems. Indicator for the fluorimetric determination of calcium in presence of Mg by complexometric titration.

Uses

Calcein is a anthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. [Cayman Chemical]

Purification Methods

Dissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]

Fluorexon Preparation Products And Raw materials

Raw materials

Preparation Products

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Fluorexon Suppliers

J & K SCIENTIFIC LTD.
Tel
400-666-7788 +86-10-82848833
Fax
86-10-82849933
Email
jkinfo@jkchemical.com;market6@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
+86-(0)21-61259100(Shanghai) +86-(0)755-86170099(ShenZhen) +86-(0)10-62670440(Beijing)
Fax
+86-(0)21-61259102(Shanghai) +86-(0)755-86170066(ShenZhen) +86-(0)10-88580358(Beijing)
Email
sh@meryer.com
Country
China
ProdList
40269
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
86-21-50328103 * 801、802、803、804 Mobile:18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15881
Advantage
69
BeiJing Hwrk Chemicals Limted
Tel
4006990298;010-57411839;0757-86311057;021-51691807
Fax
010-87653215;0757-86311057;021-55236763
Email
sales@hwrkchemical.com
Country
China
ProdList
14684
Advantage
55
Energy Chemical
Tel
021-58432009 / 400-005-6266
Fax
021-58436166-800
Email
info@energy-chemical.com
Country
China
ProdList
44025
Advantage
61
Adamas Reagent, Ltd.
Tel
400-600-9262
Email
bxy@titansci.com
Country
China
ProdList
13919
Advantage
59
Chemfun Medical Technology(Shanghai) Co., Ltd.
Tel
021-67220633 & 021-37212706
Fax
+86-21-67220638
Email
chemfun@chemfun.net
Country
China
ProdList
21812
Advantage
59
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202,021-54308259
Fax
+86-21-64545202
Email
info@hanhonggroup.com
Country
China
ProdList
43260
Advantage
64
Chengdu XiYa Chemical Technology Co., Ltd.
Tel
4008-626-111
Fax
028-84752058
Email
sale@xiyashiji.com
Country
China
ProdList
9629
Advantage
57
Tangshan Moneide Trading Co., Ltd.
Tel
+86-315-8309571
Fax
+86-315-7726572
Email
sales@moneidechem.com
Country
China
ProdList
685
Advantage
57
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View Lastest Price from Fluorexon manufacturers

career henan chemical co
Product
Fluorexon 1461-15-0
Price
US $1.00/KG
Min. Order
1G
Purity
99%
Supply Ability
100KG
Release date
2018-08-13

1461-15-0, FluorexonRelated Search:


  • 2,2',2'',2'''-(((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(Methylene))bis(azanetriyl))tetraacetic acid
  • Calcein indicator for metal determination
  • Calcein, Suitable for fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
  • Fluorescein, 2′,7′-bis[[bis(carboxymethyl)amino]methyl]-
  • Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine deriv.
  • 2,7-bis[n,n-bis(carboxymethyl)aminomethylene]- Uo-rescein
  • Acetic acid, [(3′,6′-dihydroxy-2′,7′- Uorandiyl)bis-(methylenenitrilo)]tetra-
  • Nsc 298193
  • Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine derivative
  • Fluorescein, 2 0 ,7 0 -bis[[bis(carboxymethyl) amino]methyl]-
  • Spiro[isobenzofuran-1(3 h ),9 0 -[9 h ]xanthene], Glycine derivative
  • Acetic Acid, [(3 0 ,6 0 -dihydroxy-2 0 ,7 0 -fluor- andiyl)bis(methylenenitrilo)]tetra-
  • BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN
  • CALCEIN
  • CALCEINE
  • CALCEIN, INDICATOR
  • FLUORESCEIN-2,7-BIS-METHYLIMINODIACETIC SODIUM SALT
  • FLUORESCEIN COMPLEXON
  • FLUORESCEIN COMPLEXONE
  • FLUORESCEIN-METHYLENE-IMINODIACETIC ACID
  • FLUOREXON
  • FLUOREXONE
  • FLUREXONE
  • LABOTEST-BB LT00159821
  • 2,7-BLS[BIS(CARBOXYMETHYL)AMINOMETHYL]-FLUORESCEIN
  • 3,3'-BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN
  • 3,6-DIHYDROXY-2,4-BIS-N,N'-DI-(CARBOXYMETHYL)-AMINOMETHYL FURAN
  • FLUOREXON, INDICATOR GRADE
  • CALCEIN INDICATOR FOR THE COMPLEXOMETRY
  • CalceineIndicatorGr
  • CalceineIndicatorGr(FluoresceinComplexone)
  • CalceineIndicatorGr-(FluoresceinComplexon)
  • FLUORESCEIN COMPLEXONE GR
  • Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein~Fluorexon
  • Oftasceine
  • Glycine, N,N-(3,6-dihydroxy-3-oxospiroisobenzofuran-1(3H),9-9Hxanthene-2,7-diyl)bis(methylene)bisN-(carboxymethyl)-
  • CALCEIN(RG)
  • CALCEIN HYDRATE: FOR FLUOROMETRIC DETERMINATION OF CA
  • Fluorescein di-(methylimino diacetic acid)
  • Calcein(Fluorexone)
  • Calcein [for Fluorometric Determination of Ca]
  • Fluorescein-bis(methyliminodiacetic acid), Fluorexon, Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein
  • Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, Calcein, Fluorescein Complexon
  • N,N'-[[3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-(carboxymethyl)glycine]
  • Calcein,Bis[N,Nbis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
  • Calcein, indicator for metal titration
  • Bis-[di(carboxyMethylaMinoMethyl)]flurescein sodiuM salt
  • Fluorescein-2,7-bis-MethylaMinodiacetic acid sodiuM salt
  • CALCEIN INDICATOR FOR METAL DETERMINATIO
  • 7’-diyl)bis(methylene)]bis[n-(carboxymethyl)--2
  • glycine,n,n’-[(3’,6’-dihydroxy-3-oxospiro[isobenzofuran-1(3h),9’-[9h]xanthene]
  • N,N’-[(3’,6’-dihydroxy-3-oxospiro(isobenzofuran-1(3H),9’-(9H)xanthene)-2’,7’-diyl)bis(methyGlycine
  • Fluorescein-bis(methyliminodiacetic acid)
  • oftasccine
  • CALCEINCALCEINCALCEIN
  • 1461-15-0
  • C30H26N2O13
  • C30H26N2O13xH2O