Methyl Green

Product Name: Methyl Green
CAS No.: 82-94-0
Chemical Name: Methyl Green
Synonyms: CI 42585;LIGHT GREEN;PARIS GREEN;CI NO 42585;CI NO 42590;METHYL GREEN;DOUBLE GREEN;BASIC BLUE 20;DOUBLE GREEN SF;CI BASIC BLUE 20
CBNumber: CB0389594
Molecular Formula: C26H33ClN3+
Formula Weight: 423.02
MOL File: 82-94-0.mol

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Methyl Green Property

Water Solubility: Soluble in water
solubility: Methanol (Slightly), Water (Slightly)
Colour Index: 42585
form: Liquid
color: Clear blue-green
PH Range: 0.1(yellow)-2.3(blue)
Stability:: Hygroscopic
CAS DataBase Reference: 82-94-0
EPA Substance Registry System: C.I. Basic Blue 20 (82-94-0)

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Safety

HS Code: 3204.13.8000

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H413May cause long lasting harmful effects to aquatic life

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 198080
Product name: Methyl Green
Purity: zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?%
Packaging: 10g
Price: $130
Updated: 2021/12/16

Sigma-Aldrich

Product number: 198080
Product name: Methyl Green
Purity: zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?%
Packaging: 50g
Price: $660
Updated: 2021/12/16

TCI Chemical

Product number: M0498
Product name: Methyl Green
Packaging: 25g
Price: $214
Updated: 2024/03/01

TRC

Product number: B400378
Product name: [4-[[4-(Dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethylazaniumDichloride
Packaging: 250mg
Price: $45
Updated: 2021/12/16

Chem-Impex

Product number: 38658
Product name: Methylgreen,CI42585
Packaging: 25G
Price: $234.12
Updated: 2021/12/16

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Methyl Green Chemical Properties,Usage,Production

Description

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Uses

Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.

Definition

ChEBI: Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.

Preparation

chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction

Properties and Applications

green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.

Standard( Cotton )	Light Fastness	Persperation Fastness		Ironing Fastness		Soaping
Standard( Cotton )	Light Fastness	Fading	Stain	Fading	Stain	Fading	Stain
C	1					2

Purification Methods

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]

Methyl Green Preparation Products And Raw materials

Raw materials

Preparation Products

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Methyl Green Suppliers

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82-94-0, Methyl GreenRelated Search:

METHYL GREEN 23S,METHYL GREEN,METHYL GREEN GA Melb Health-RMH 2% Methyl Green Chlorofom Wash METHYL GREEN,GLYCEROL /METHYL GREEN,METHYL GREEN ZINC CHLORIDE DOUBLE SALT,CI NO 42585 Deoxyribonucleic acid-methyl green complex, DNA-Methyl Green,DEOXYRIBONUCLEIC ACID METHYL GREEN,DNA-METHYL GREEN Methyl Green zinc chloride salt DNASE TEST AGAR WITH METHYL GREEN PYRONIN METHYL GREEN,METHYL GREEN-PYRONIN METHYL GREEN (C.I. No 42590 ),Methyl Green, 0.1% w/v aq. soln.,METHYL GREEN 23S,METHYL GREEN,METHYL GREEN GA Pyronine methyl green METHYL GREEN (C.I. 42590) BIOCHEMICA PYRONIN METHYL GREEN (PAPPENHEIM),METHYL GREEN PYRONINE MPS (Methyl Green Pyronine Stilbene) METHYL GREEN OO,CI NO 42585 GLYCEROL (50 %) / METHYL GREEN (0.6 %) METHYL GREEN - SOLUTION Deoxyribonuclease (DNase) Test Agar w/Methyl Green (Powder) tetrachloroplatinate dianion-methyl green complex Deoxyribonucleic acid-methyl green standardization set

PARIS GREEN

n,n-trimethyl-n-1-ylidene]methyl]-dichloride

METHYL GREEN

METHYL GREEN ZINC CHLORIDE DOUBLE SALT

Benzenaminium, 4-4-(dimethylamino)phenyl4-(dimethyliminio)-2,5-cyclohexadien-1-ylidenemethyl-N,N,N-trimethyl-, dichloride

Methyl Green Dichloride

HEPTAMETHYL-P-ROSANILINE CHLORIDE

GLYCEROL /METHYL GREEN

LIGHT GREEN

CI BASIC BLUE 20

CI 42585

CI NO 42585

CI NO 42590

DOUBLE GREEN

DOUBLE GREEN SF

BASIC BLUE 20

4-[[4-(Dimethylamino)phenyl][4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene]methyl]-N,N,N-trimethylanilinium dichloride

[4-[(4-dimethylaminophenyl)-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethylazanium dichloride

[4-[(4-dimethylaminophenyl)-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethyl-azanium dichloride

[4-[(4-dimethylaminophenyl)-(4-dimethyliminiocyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethyl-ammonium dichloride

Methyl Green zinc ch

C.I. 42585 or 42590

4-{[4-(DiMethylaMino)phenyl][4-(diMethyliMinio)-2,5-cyclohexadien-1-ylidene]Methyl}-N,N,N-triMethylaniliniuM dichloride

Methyl green indicator (0.1-2.0)

C.I.Basic Blue 20:C.I.42585

Basic blue 20 (C.l. 42585)

82-94-0

C26H33N32Cl