Methyl Green

Methyl Green Property

storage temp. 

2-8°C, sealed storage, away from moisture and light

Water Solubility 

Soluble in water

solubility 

Methanol (Slightly), Water (Slightly)

Colour Index 

42585

form 

Liquid

color 

Clear blue-green

PH Range

0.1(yellow)-2.3(blue)

Stability:

Hygroscopic

CAS DataBase Reference

82-94-0

EPA Substance Registry System

C.I. Basic Blue 20 (82-94-0)

Safety

HS Code 

3204.13.8000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H413May cause long lasting harmful effects to aquatic life

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

Methyl Green Chemical Properties,Usage,Production

Description

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Chemical Properties

Methyl Green is a green microcrystalline or bright green powder with metallic luster. It is soluble in water and shows blue-green color. It is a basic dye, it is easy to combine with the high degree of polymerization of DNA to show green. Also known as double green SF. green crystals with a golden luster, or light green powder. Soluble in water, blue-green color. Slightly soluble in ethanol, insoluble in ether, pentanol. Methyl green exists in two forms. Ⅰ formula: molecular formula C26H33Cl2N3. molecular weight 458.49. green crystal, with golden yellow luster or light green powder. Soluble in water, slightly soluble in ethanol, insoluble in ether. Usually the complex salt of zinc chloride. Ⅱ formula: molecular formula C27H35BrClN3. molecular weight 516.98. green powder, soluble in water greenish blue. The solution is yellow at pH 0.2 and blue at pH 1.8.

Uses

Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.

Definition

ChEBI: Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.

Preparation

chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction

Properties and Applications

green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.

Purification Methods

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]