Methyl Green
- Product Name
- Methyl Green
- CAS No.
- 82-94-0
- Chemical Name
- Methyl Green
- Synonyms
- CI 42585;LIGHT GREEN;PARIS GREEN;CI NO 42585;CI NO 42590;METHYL GREEN;DOUBLE GREEN;BASIC BLUE 20;DOUBLE GREEN SF;CI BASIC BLUE 20
- CBNumber
- CB0389594
- Molecular Formula
- C26H33ClN3+
- Formula Weight
- 423.02
- MOL File
- 82-94-0.mol
Methyl Green Property
- Water Solubility
- Soluble in water
- solubility
- Methanol (Slightly), Water (Slightly)
- Colour Index
- 42585
- form
- Liquid
- color
- Clear blue-green
- PH Range
- 0.1(yellow)-2.3(blue)
- Stability:
- Hygroscopic
- CAS DataBase Reference
- 82-94-0
- EPA Substance Registry System
- C.I. Basic Blue 20 (82-94-0)
Safety
- HS Code
- 3204.13.8000
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
H413May cause long lasting harmful effects to aquatic life
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P321Specific treatment (see … on this label).
P332+P313IF SKIN irritation occurs: Get medical advice/attention.
P362Take off contaminated clothing and wash before reuse.
N-Bromosuccinimide Price
- Product number
- 198080
- Product name
- Methyl Green
- Purity
- zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?%
- Packaging
- 10g
- Price
- $130
- Updated
- 2021/12/16
- Product number
- 198080
- Product name
- Methyl Green
- Purity
- zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?%
- Packaging
- 50g
- Price
- $660
- Updated
- 2021/12/16
- Product number
- M0498
- Product name
- Methyl Green
- Packaging
- 25g
- Price
- $214
- Updated
- 2024/03/01
- Product number
- B400378
- Product name
- [4-[[4-(Dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethylazaniumDichloride
- Packaging
- 250mg
- Price
- $45
- Updated
- 2021/12/16
- Product number
- 38658
- Product name
- Methylgreen,CI42585
- Packaging
- 25G
- Price
- $234.12
- Updated
- 2021/12/16
Methyl Green Chemical Properties,Usage,Production
Description
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.
Uses
Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.
Definition
ChEBI: Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.
Preparation
chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction
Properties and Applications
green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.
| Standard( Cotton ) | Light Fastness | Persperation Fastness | Ironing Fastness | Soaping | |||
| Fading | Stain | Fading | Stain | Fading | Stain | ||
| C | 1 | 2 | |||||
Purification Methods
Crystallise the dye from hot water. [Beilstein 13 IV 2286.]