RED BLOOD CELL LYSING BUFFER HYBRI-MAX

Product Name
RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Chemical Name
RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Synonyms
RBC Lysing Buffer;Red Cell Lysis Buffer;RED BLOOD CELL LYSING BUFFER HYBRI-MAX
CBNumber
CB0566979
Formula Weight
0
MOL File
Mol file
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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Property

form 
liquid
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
R7757
Product name
Red Blood Cell Lysing Buffer Hybri-Max™
Purity
liquid, sterile-filtered, suitable for hybridoma
Packaging
100ML
Price
$28.1
Updated
2023/06/20
American Custom Chemicals Corporation
Product number
BBF0000082
Product name
RBC LYSING BUFFER
Purity
95.00%
Packaging
5MG
Price
$497.08
Updated
2021/12/16
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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Chemical Properties,Usage,Production

Uses

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

RED BLOOD CELL LYSING BUFFER HYBRI-MAX Preparation Products And Raw materials

Raw materials

Preparation Products

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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Suppliers

Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973130 4009686088
Email
3193328036@qq.com
Country
China
ProdList
29785
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68
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4727
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58
Shanghai Macklin Biochemical Co.,Ltd.
Tel
15221275939 15221275939
Fax
021-50706099
Email
shenlinxing@macklin.cn
Country
China
ProdList
16166
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Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973186 4009686088
Email
3193328036@qq.com
Country
China
ProdList
18338
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68
Beijing Jin Ming Biotechnology Co., Ltd.
Tel
010-60605840 18892239720
Fax
010-60605840
Email
psaitong@jm-bio.com
Country
China
ProdList
12306
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58
Shanghai Kanglang Biotechnology Co., Ltd.
Tel
021-61998208 18217752821
Fax
021-37680378
Email
sales@klbio.cn
Country
China
ProdList
9618
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58
Condice Chemical (Hubei) Co., Ltd.
Tel
18064039730
Email
3127859172@qq.com
Country
China
ProdList
2828
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58
Wuhan Chemstan Biotechnology Co., Ltd.
Tel
027-65317797 15926423062
Fax
QQ:422450190
Email
422450190@qq.com
Country
China
ProdList
10424
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58
Shanghai Universal Biotech Co.,Ltd
Tel
13774214275
Email
zhouzz@univ-bio.com
Country
China
ProdList
24994
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58
Guangdong wengjiang Chemical Reagent Co., Ltd.
Tel
0751-2815987 13927875076
Fax
0751-2828607
Email
3007432263@qq.com
Country
China
ProdList
9969
Advantage
58