Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin
- Product Name
- Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin
- Chemical Name
- Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin
- Synonyms
- Sulfo-Cy5-PEG3-Biotin;Sulfo-Cyanine5-PEG3-Biotin;Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin
- CBNumber
- CB110653765
- Formula Weight
- 0
- MOL File
- Mol file
Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Property
- solubility
- good in water, DMF, DMSO
- Appearance
- dark blue solid
Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Chemical Properties,Usage,Production
Description
sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.
Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.
sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.
The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.
Abs/Em Maxima
646/662 nm
Fluoresene quantum yield
0.28
Extinction Coefficient
271000
CF260
0.04
CF280
0.04
Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Preparation Products And Raw materials
Raw materials
Preparation Products
Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Suppliers
- Tel
- 86-0573-85866609 13325730825
- Fax
- 0573-85866609
- 945717149@qq.com
- Country
- China
- ProdList
- 8593
- Advantage
- 58
- Tel
- 029-68881181 13619217766
- Fax
- 029-68881181
- 3495811982@qq.com
- Country
- China
- ProdList
- 2803
- Advantage
- 58
- Tel
- 400-400-6966-012-012 17778955912
- 2957605606@qq.com
- Country
- China
- ProdList
- 2535
- Advantage
- 58
- Tel
- 18889736353
- order@lumiprobe.com
- Country
- China
- ProdList
- 586
- Advantage
- 58
- Tel
- 65-6980-8108 65698081
- info@probioscience.org
- Country
- China
- ProdList
- 6014
- Advantage
- 58
- Tel
- 4000099791
- Country
- CHINA
- ProdList
- 181
- Advantage
- 58