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Evans Blue

Product Name
Evans Blue
CAS No.
314-13-6
Chemical Name
Evans Blue
Synonyms
t1824;evablin;CI 23860;C.I.24075;DiazoBlue;blue Evan;Evans Blu;diazobleu;Evens Blue;EVANS BLUE
CBNumber
CB1161546
Molecular Formula
C34H29N6NaO14S4
Formula Weight
896.86
MOL File
314-13-6.mol
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Evans Blue Property

storage temp. 
Store at RT.
solubility 
DMSO (Slightly), Water (Slightly)
Colour Index 
23860
form 
Powder/Solid
color 
Dark Brown
Water Solubility 
280 g/L (20 ºC)
Merck 
14,3905
Stability:
Stable. Incompatible with strong reducing agents, strong oxidizing agents.
CAS DataBase Reference
314-13-6
IARC
3 (Vol. 8, Sup 7) 1987
EPA Substance Registry System
C.I. Direct Blue 53 (314-13-6)
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Safety

Hazard Codes 
T,F
Risk Statements 
45-63-11-61-20/21/22
Safety Statements 
53-45-36/37-16-36/37/39
WGK Germany 
3
RTECS 
QJ6440000
TSCA 
Yes
HS Code 
32129000
Toxicity
LD50 intraperitoneal in mouse: 340mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H350May cause cancer

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
E2129
Product name
Evans Blue
Purity
Dye content ≥75%
Packaging
10g
Price
$68
Updated
2024/03/01
Sigma-Aldrich
Product number
E2129
Product name
Evans Blue
Purity
Dye content ≥75%
Packaging
50g
Price
$270
Updated
2024/03/01
TCI Chemical
Product number
E0197
Product name
Evans Blue
Purity
>80.0%(HPLC)
Packaging
25g
Price
$55
Updated
2024/03/01
Alfa Aesar
Product number
A16774
Product name
Evans Blue
Packaging
10g
Price
$53.2
Updated
2024/03/01
Alfa Aesar
Product number
A16774
Product name
Evans Blue
Packaging
50g
Price
$174
Updated
2024/03/01
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Evans Blue Chemical Properties,Usage,Production

Chemical Properties

Evans Blue is a blue crystal with a green metallic luster. It is very soluble in water, slightly soluble in ethanol, and almost insoluble in benzene, chloroform, and ether. It is soluble in acids and alkalis. It is hygroscopic, inducing moisture. It changes color when the pH is close to 10.

Uses

Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology.

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.

Definition

ChEBI: Evans blue is an organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppress background autofluorescence. It has a role as a histological dye, a fluorochrome, a teratogenic agent and a sodium channel blocker. It contains an Evans blue(4-).

General Description

Blue crystals with a greenish-bronze luster or a black powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.

Reactivity Profile

Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents.

Fire Hazard

Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible.

Biological Activity

Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist.

Safety Profile

Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it

in vitro

evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2].

IC 50

220 and 150 nm for glurl and giur6, respectively.

storage

Room temperature

Staining Procedures

Evans Blue (3,7-Diamino-5-phenylphenazinium chloride) is used as a vital stain. It is used in a 0.05-0.25% aqueous solution for 5 minutes. Living cells remain unstained, while dead cells become blue.?

Properties and Applications

dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.

Standard Acid Resistance Alkali Resistance Light Fastness Soaping Water
Fading Stain Fading Stain
ISO 5 4-5 1-2 2 2

References

[1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103.
[2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71.

Evans Blue Preparation Products And Raw materials

Raw materials

Preparation Products

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Evans Blue Suppliers

Americas 1 Belgium 1 Canada 1 China 179 Europe 4 France 2 Germany 5 India 41 Japan 4 Poland 1 Russia 1 South Korea 1 Switzerland 2 United Kingdom 11 United States 46 Global 300
NANTONG XINHE BIOTECH CO.,LTD
Tel
13813727166，15370460228; 13813727166
Email
sale_xinhe@outlook.com
Country
China
ProdList
107
Advantage
58
BIOISCO(Lianyungang, Jiangsu) Biotechnology Co., Ltd
Tel
18082049515
Email
2757382053@qq.com
Country
China
ProdList
2148
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8415
Advantage
55
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44689
Advantage
61
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
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View Lastest Price from Evans Blue manufacturers

Zhuozhou Wenxi import and Export Co., Ltd
Product
Direct Blue 53 314-13-6
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-08-11
Career Henan Chemical Co
Product
Direct Blue 53 314-13-6
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
100kg
Release date
2019-07-09

314-13-6, Evans BlueRelated Search:

Azobenzene Dimethyl sulfoxide ETHANE Dimethyl carbonate Dimethyl fumarate Biphenyl Diisopropyl azodicarboxylate tert-Butylchlorodiphenylsilane Dichlorodiphenylsilane 2,2'-Azodi(2-methylbutyronitrile) Diphenylsilane Chlorodiphenylphosphine Azodicarbonamide N,N-Dimethylformamide Diphenylphosphine Dacthal Dimethyl ether 4-AMINOAZOBENZENE

  • EVANS BLUE C.I. No 23860
  • EvansBlue,99%
  • DiazoBlue
  • 1,3-Naphthalenedisulfonic acid, 6,6-(3,3-dimethyl1,1-biphenyl-4,4-diyl)bis(azo)bis4-amino-5-hydroxy-, tetrasodium salt
  • 4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyl tetrasodium salt
  • 6,6'-[(3,3'-Dimethyl-4,4'-biphenylylene)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid] tetrasodium salt
  • 6,6'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis(4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid disodium)
  • 3-[[4'-[(4-Amino-2-methyl-5-methoxyphenyl)azo]-3,3'-dimethoxy-1,1'-biphenyl-4-yl]azo]-4-hydroxynaphthalene-1,5-disulfonic acid disodium salt
  • C.I.24075
  • 6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT
  • EVANS BLUE TETR
  • Evans Blue,Direct Blue 53
  • EVANS BLUE COUNTERSTAIN
  • EVANS BLUE, FOR MICROSCOPY
  • blue Evan
  • evans blue (C.I. 23860)
  • evans blue fluka
  • Evens Blue
  • tetrasodium 6,6-((3,3-dimethyl-(1,1-biphenyl-4,4diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate)
  • tetrasodium 6,6'-((3,3'-dimethyl-(1,1'-biphenyl-4,4'diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate)
  • Evans Blue 5GR
  • EVANS BLUE
  • EVAN'S BLUE
  • EVANS BLUE PURE
  • EVANS BLUE TETRASODIUM SALT
  • GEIGY BLUE
  • DIRECT BLUE 53
  • CI NO 23860
  • CI 23860
  • 1,3-naphthalenedisulfonicacid,6,6’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo
  • 1,3-naphthalenedisulfonicacid,6,6’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)b
  • 1,3-Naphthalenedisulfonicacid,6,6’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(4-amino-5-hydroxy-,tetrasodiumsalt
  • 4,4’-bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3’-bitolyl,tetrasodi
  • 4,4’-bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3’-bitolyl,tetrasod
  • 4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3’-bitolyl,tetrasod
  • 4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3’-bitolyl,tetraso
  • 4,4’-bis-[7-(1-Amino-8-hydroxy-2,4-disulpho-naphthyl)azo]-3,3-bitolylsodiumsalt
  • Evans Blu
  • 6,6’-[3,3’-Dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)-bis-[4-amino-5-hydroxy-1,3-naphthale-nedisulfo]
  • azovanblue
  • blekitevansa
  • C.I.DirectBlue5,3-tetrasodiumsalt
  • c.i.directblue53
  • c.i.directblue53,tetrasodiumsalt
  • diamineskyblueff
  • diazobleu
  • diazolpurebluebf
  • directblue53(evan’s
  • directblue53(evan’sblue)
  • dyeevansblue
  • evablin
  • Evans blue, Dye content: &bsim:85%
  • 6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT
  • 3,3'-DITOLYL-4,4'-BIS[7-AZO-1-AMINO-8-NAPHTHOL-2,4-(DISODIUM)DISULFONATE]
  • evansblue,sodiumsalt
  • evansbluedye
  • geigy-blau536
  • geigyblue536,med