Evans Blue

Product Name: Evans Blue
CAS No.: 314-13-6
Chemical Name: Evans Blue
Synonyms: t1824;evablin;CI 23860;C.I.24075;DiazoBlue;blue Evan;Evans Blu;diazobleu;Evens Blue;EVANS BLUE
CBNumber: CB1161546
Molecular Formula: C34H29N6NaO14S4
Formula Weight: 896.86
MOL File: 314-13-6.mol

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Evans Blue Property

storage temp.: Store at RT.
solubility: DMSO (Slightly), Water (Slightly)
Colour Index: 23860
form: Powder/Solid
color: Dark Brown
Water Solubility: 280 g/L (20 ºC)
Merck: 14,3905
Stability:: Stable. Incompatible with strong reducing agents, strong oxidizing agents.
CAS DataBase Reference: 314-13-6
IARC: 3 (Vol. 8, Sup 7) 1987
EPA Substance Registry System: C.I. Direct Blue 53 (314-13-6)

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Safety

Hazard Codes: T,F
Risk Statements: 45-63-11-61-20/21/22
Safety Statements: 53-45-36/37-16-36/37/39
WGK Germany: 3
RTECS: QJ6440000
TSCA: Yes
HS Code: 32129000
Toxicity: LD50 intraperitoneal in mouse: 340mg/kg

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H350May cause cancer

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: E2129
Product name: Evans Blue
Purity: Dye content ≥75%
Packaging: 10g
Price: $68
Updated: 2024/03/01

Sigma-Aldrich

Product number: E2129
Product name: Evans Blue
Purity: Dye content ≥75%
Packaging: 50g
Price: $270
Updated: 2024/03/01

TCI Chemical

Product number: E0197
Product name: Evans Blue
Purity: >80.0%(HPLC)
Packaging: 25g
Price: $55
Updated: 2024/03/01

Alfa Aesar

Product number: A16774
Product name: Evans Blue
Packaging: 10g
Price: $53.2
Updated: 2024/03/01

Alfa Aesar

Product number: A16774
Product name: Evans Blue
Packaging: 50g
Price: $174
Updated: 2024/03/01

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Evans Blue Chemical Properties,Usage,Production

Chemical Properties

Evans Blue is a blue crystal with a green metallic luster. It is very soluble in water, slightly soluble in ethanol, and almost insoluble in benzene, chloroform, and ether. It is soluble in acids and alkalis. It is hygroscopic, inducing moisture. It changes color when the pH is close to 10.

Uses

Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology.

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.

Definition

ChEBI: Evans blue is an organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppress background autofluorescence. It has a role as a histological dye, a fluorochrome, a teratogenic agent and a sodium channel blocker. It contains an Evans blue(4-).

General Description

Blue crystals with a greenish-bronze luster or a black powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.

Reactivity Profile

Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents.

Fire Hazard

Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible.

Biological Activity

Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist.

Safety Profile

Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it

in vitro

evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2].

IC 50

220 and 150 nm for glurl and giur6, respectively.

storage

Room temperature

Staining Procedures

Evans Blue (3,7-Diamino-5-phenylphenazinium chloride) is used as a vital stain. It is used in a 0.05-0.25% aqueous solution for 5 minutes. Living cells remain unstained, while dead cells become blue.?

Properties and Applications

dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	5	4-5	1-2	2		2

References

[1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103.
[2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71.

Evans Blue Preparation Products And Raw materials

Raw materials

Preparation Products

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Evans Blue Suppliers

Americas 1 Belgium 1 Canada 1 China 179 Europe 4 France 2 Germany 5 India 42 Japan 4 Poland 1 Russia 1 South Korea 1 Switzerland 2 United Kingdom 11 United States 46 Global 301

Apollo Scientific Ltd.

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VWR International

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View Lastest Price from Evans Blue manufacturers

Zhuozhou Wenxi import and Export Co., Ltd

Product: Direct Blue 53 314-13-6
Price: US $15.00-10.00/KG
Min. Order: 1KG
Purity: 99%+ HPLC
Supply Ability: Monthly supply of 1 ton
Release date: 2021-08-11

Career Henan Chemical Co

Product: Direct Blue 53 314-13-6
Price: US $1.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 100kg
Release date: 2019-07-09

314-13-6, Evans BlueRelated Search:

Azobenzene Dimethyl sulfoxide ETHANE Dimethyl carbonate Dimethyl fumarate Biphenyl Diisopropyl azodicarboxylate tert-Butylchlorodiphenylsilane Dichlorodiphenylsilane 2,2'-Azodi(2-methylbutyronitrile) Diphenylsilane Chlorodiphenylphosphine Azodicarbonamide N,N-Dimethylformamide Diphenylphosphine Dacthal Dimethyl ether 4-AMINOAZOBENZENE

EVANS BLUE C.I. No 23860

EvansBlue,99%

DiazoBlue

1,3-Naphthalenedisulfonic acid, 6,6-(3,3-dimethyl1,1-biphenyl-4,4-diyl)bis(azo)bis4-amino-5-hydroxy-, tetrasodium salt

4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyl tetrasodium salt

6,6'-[(3,3'-Dimethyl-4,4'-biphenylylene)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid] tetrasodium salt

6,6'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis(4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid disodium)

3-[[4'-[(4-Amino-2-methyl-5-methoxyphenyl)azo]-3,3'-dimethoxy-1,1'-biphenyl-4-yl]azo]-4-hydroxynaphthalene-1,5-disulfonic acid disodium salt

C.I.24075

6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT

EVANS BLUE TETR

Evans Blue,Direct Blue 53

EVANS BLUE COUNTERSTAIN

EVANS BLUE, FOR MICROSCOPY

blue Evan

evans blue (C.I. 23860)

evans blue fluka

Evens Blue

tetrasodium 6,6-((3,3-dimethyl-(1,1-biphenyl-4,4diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate)

tetrasodium 6,6'-((3,3'-dimethyl-(1,1'-biphenyl-4,4'diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate)

Evans Blue 5GR

EVANS BLUE

EVAN'S BLUE

EVANS BLUE PURE

EVANS BLUE TETRASODIUM SALT

GEIGY BLUE

DIRECT BLUE 53

CI NO 23860

CI 23860

1,3-naphthalenedisulfonicacid,6,6’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo

1,3-naphthalenedisulfonicacid,6,6’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)b

1,3-Naphthalenedisulfonicacid,6,6’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(4-amino-5-hydroxy-,tetrasodiumsalt

4,4’-bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3’-bitolyl,tetrasodi

4,4’-bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3’-bitolyl,tetrasod

4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3’-bitolyl,tetrasod

4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3’-bitolyl,tetraso

4,4’-bis-[7-(1-Amino-8-hydroxy-2,4-disulpho-naphthyl)azo]-3,3-bitolylsodiumsalt

Evans Blu

6,6’-[3,3’-Dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)-bis-[4-amino-5-hydroxy-1,3-naphthale-nedisulfo]

azovanblue

blekitevansa

C.I.DirectBlue5,3-tetrasodiumsalt

c.i.directblue53

c.i.directblue53,tetrasodiumsalt

diamineskyblueff

diazobleu

diazolpurebluebf

directblue53(evan’s

directblue53(evan’sblue)

dyeevansblue

evablin

Evans blue, Dye content: &bsim:85%

6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT

3,3'-DITOLYL-4,4'-BIS[7-AZO-1-AMINO-8-NAPHTHOL-2,4-(DISODIUM)DISULFONATE]

evansblue,sodiumsalt

evansbluedye

geigy-blau536

geigyblue536,med