Luminol Property

Melting point:

>300 °C (lit.)

Boiling point:

309.07°C (rough estimate)

Density 

1.3393 (rough estimate)

refractive index 

1.6500 (estimate)

storage temp. 

Store below +30°C.

solubility 

Insoluble in water, soluble in dimethyl sulfoxide.

pka

(Calcd.) 10.50 ± 0.20;;0.58 ± 0.20(at 25℃)

form 

Powder

color 

Dark green to dark blue-purple

PH Range

NonH uorescence (6.0) to blue H uorescence (7.0)

Water Solubility 

<0.1 g/100 mL at 19 ºC

λmax

425nm

Merck 

14,5600

BRN 

383929

Stability:

Stable. Combustible. Incompatible with strong oxidizing agents, strong acids, strong bases, strong reducing agents. Emits light on reaction with oxidizers.

Major Application

Night vision devices, identification of product forgeries, determination of nitride in food, analytical chemistry, pharmaceuticals, iodine in biological materials, evaluating phagocytes, detecting tumors, DNA, biosensors, assay of fungi, bacteria, yeasts, assay of lipase activity

InChIKey

HWYHZTIRURJOHG-UHFFFAOYSA-N

LogP

-0.245 (est)

CAS DataBase Reference

521-31-3(CAS DataBase Reference)

NIST Chemistry Reference

3-Aminophthalhydrazide(521-31-3)

EPA Substance Registry System

Luminol (521-31-3)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-22-20/21/22

Safety Statements 

26-36/37-37/39-36

WGK Germany 

3

RTECS 

TH8890060

F 

10-23

TSCA 

Yes

HS Code 

29339990

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Luminol Chemical Properties,Usage,Production

Chemical fluorescent molecules

Luminol is an organic compound which, when oxidized, emits light — a phenomenon known as chemiluminescence.  This is similar to the reactions that fireflies uses to emit light, and to those used in "glow-sticks" and some roadside emergency lights. In this reaction, a small amount of luminol (3-aminophthalhydrazide or 5-amino-2,3-dihydro- 1,4-phthalazinedione) is dissolved in a basic aqueous solution, which also contains a small amount of copper(II) sulfate.  To this solution is added a solution of a mild oxidizing agent, which is 0.3% hydrogen peroxide in the demonstration below.  (Bleach is also used in some recipes as the oxidizing agent.)  The reaction is believed to occur by the following mechanism:

Chemiluminescence method

Luminol chemiluminescence has been trialed for estimating the PMI from skeletal remains. Luminol methods are based on the reaction between luminol and hydrogen peroxide which is catalyzed by iron in hemoglobin found in fluids and tissues from bodies. This reaction results in a chemiluminescence light which can be measured by various means .

Chemical properties

Yellow crystalline powder, Easily soluble in lye, soluble in dilute acid but almost insoluble in water and hardly soluble in alcohol. When its neutral or weak acidic solution is exposed to ultraviolet light, it exhibits strongly bright blue fluorescence. Melting point: 329-332 &deg; C

Uses

1.    It can be used as chemical analysis reagents, indicators.
2.    It can be used for chemiluminescence analysis such as measurement of metal cations, blood and glucocorticoids

Hazards & Safety Information

Category Toxic substances
Toxic grading poisoning
Acute toxicity Oral – rat; LD50: > 500 mg/kg
Flammability and Hazardous properties it is combustible with combustion producing toxic nitrogen oxide fumes
Storage and transportation characteristics warehouse: ventilated, low temperature and dry
Fire extinguishing agent dry powder, foam, sand, carbon dioxide, mist water

Chemical Properties

yellow crystals or beige powder

Uses

Luminol is a chemiluminescent probe that has been used to detect myeloperoxidase-mediated oxidative events in granulocytes and for chemiluminescence analysis of metal cations and blood.

Uses

Luminol is used in the detection of copper, iron, peroxides and cyanides. It exhibits chemiluminescence and utilized to measure opsonic and phagocytic function. It acts as a biological sensor and involved in the detection of polymorphonuclear leukocytes response in a patient with a myeloperoxidase deficiency. Further, it is used as a forensic test for blood.

Uses

Detection of copper, iron, peroxides, cyanides.

General Description

Yellow crystals or light beige powder.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Oxidation of 3-Aminophthalhydrazide is accompanied by a striking emission of light. . .

Health Hazard

ACUTE/CHRONIC HAZARDS: When heated to decomposition 3-Aminophthalhydrazide emits toxic fumes.

Fire Hazard

Flash point data for 3-Aminophthalhydrazide are not available, but 3-Aminophthalhydrazide is probably combustible.

Synthesis

The synthesis of Luminol is as follows: To a reaction tube, add 140 mg of 3-nitrophthalhydrazide and 1.0 mL of 3 M sodium hydroxide solution. Stir with a rod, and to the resulting deep brown-red solution add 0.6 g of sodium hydrosulfite dihydrate (Na2S2O4 · 2 H2O, MW 210.2). Wash down the sides of the tube with a small amount of water. Heat to a gentle boil and keep the tube hot for 5 minutes. During this time some product may begin to crystallize. Add 0.4 mL of acetic acid, cool the tube in cold water, and stir. Collect the light-yellow product Luminol by suction filtration.

storage

Room temperature

Purification Methods

Dissolve luminol in KOH solution, treat with Norit (charcoal), filter and precipitate it with conc HCl. [Hardy et al. Talanta 24 297 1977.] Store it in the dark in an inert atmosphere, because its structure changes during its luminescence. It has been recrystallised from 0.1M KOH [Merenyi et al. J Am Chem Soc 108 77716 1986]. [Beilstein 25 II 389, 25 III/IV 4192.]