COELENTERAZINE H
- Product Name
- COELENTERAZINE H
- CAS No.
- 50909-86-9
- Chemical Name
- COELENTERAZINE H
- Synonyms
- COEL;CLZ-H;CLZN-H;ENTERAZINE H;Colenterazine H;COELENTERAZINE H;H-coelenterazine;Renilla Luciferin;LUCIFERIN (RENILLA);2-DEOXYCOELENTERAZINE
- CBNumber
- CB1304820
- Molecular Formula
- C26H21N3O2
- Formula Weight
- 407.46
- MOL File
- 50909-86-9.mol
COELENTERAZINE H Property
- Melting point:
- >200℃
- Boiling point:
- 593.5±60.0 °C(Predicted)
- Density
- 1.26±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in methanol, ethanol
- pka
- 10.50±0.30(Predicted)
- form
- solid
- color
- Yellow
- λmax
- 437nm
- Stability:
- Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
- Biological Applications
- Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- C3230
- Product name
- Coelenterazine h
- Purity
- solid
- Packaging
- 50μg
- Price
- $203
- Updated
- 2024/03/01
- Product number
- 16894
- Product name
- Coelenterazine h
- Purity
- ≥95%
- Packaging
- 500μg
- Price
- $183
- Updated
- 2024/03/01
- Product number
- 16894
- Product name
- Coelenterazine h
- Purity
- ≥95%
- Packaging
- 1mg
- Price
- $345
- Updated
- 2024/03/01
- Product number
- C636025
- Product name
- Coelenterazine h
- Packaging
- 1mg
- Price
- $285
- Updated
- 2021/12/16
- Product number
- CHM0007260
- Product name
- COELENTERAZINE H
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $496.66
- Updated
- 2021/12/16
COELENTERAZINE H Chemical Properties,Usage,Production
Description
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
Uses
Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.
Definition
ChEBI: Renilla luciferin is a member of phenols and an imidazopyrazine. It has a role as a luciferin. It derives from a hydride of an imidazo[1,2-a]pyrazine.
General Description
Coelenterazine is an imidazopyrazinone derivative. It is a substrate for bioluminescence in many luminous marine organisms.
Biochem/physiol Actions
10 times higher luminescence intensity than native coelenterazine.
References
1) Shimomura?et al. (1989),?Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J.,?261?913 2) Nakajima-Shimada?et al.?(1991),?Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA,?88?6878 3) Inouye?et al. (1992),?Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem.,?201?114