ChemicalBook > CAS DataBase List > 2-Nitrophenyl-beta-D-galactopyranoside

2-Nitrophenyl-beta-D-galactopyranoside

Product Name
2-Nitrophenyl-beta-D-galactopyranoside
CAS No.
369-07-3
Chemical Name
2-Nitrophenyl-beta-D-galactopyranoside
Synonyms
ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
CBNumber
CB1366392
Molecular Formula
C12H15NO8
Formula Weight
301.25
MOL File
369-07-3.mol
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2-Nitrophenyl-beta-D-galactopyranoside Property

Melting point:
186-193 °C (dec.)
alpha 
-70 º (c=1, H2O)
Boiling point:
442.39°C (rough estimate)
Density 
1.3709 (rough estimate)
refractive index 
-68.8 ° (C=1, H2O)
storage temp. 
2-8°C
solubility 
DMSO (Slightly), Water (Slightly, Heated, Sonicated)
pka
12.69±0.70(Predicted)
form 
Powder
color 
White to very slightly yellow
optical activity
[α]20/D 70±1°, c = 1% in H2O
Water Solubility 
Soluble in water.
BRN 
92207
InChIKey
KUWPCJHYPSUOFW-RMPHRYRLSA-N
CAS DataBase Reference
369-07-3(CAS DataBase Reference)
NIST Chemistry Reference
Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
F 
3-10-21
HS Code 
29400090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
1g
Price
$54.7
Updated
2024/03/01
Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
5G
Price
$208
Updated
2024/03/01
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
1G
Price
$40.3
Updated
2022/05/15
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
5G
Price
$142
Updated
2022/05/15
TCI Chemical
Product number
N0418
Product name
2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
Purity
>98.0%(HPLC)
Packaging
1g
Price
$30
Updated
2021/12/16
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2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Usage,Production

Description

2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.

Chemical Properties

White Crystalline Solid

Uses

2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.

Definition

ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.

General Description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Purification Methods

Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]

2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from 2-Nitrophenyl-beta-D-galactopyranoside manufacturers

Hebei Duling International Trade Co. LTD
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $50.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
1
Release date
2022-10-18
Hebei Yanxi Chemical Co., Ltd.
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $40.00-40.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10 tons
Release date
2023-09-25
WUHAN FORTUNA CHEMICAL CO., LTD
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
98.0%
Supply Ability
500kg/month
Release date
2023-01-09

369-07-3, 2-Nitrophenyl-beta-D-galactopyranosideRelated Search:

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