ChemicalBook > CAS DataBase List > 2-Nitrophenyl-beta-D-galactopyranoside

2-Nitrophenyl-beta-D-galactopyranoside

Product Name
2-Nitrophenyl-beta-D-galactopyranoside
CAS No.
369-07-3
Chemical Name
2-Nitrophenyl-beta-D-galactopyranoside
Synonyms
ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
CBNumber
CB1366392
Molecular Formula
C12H15NO8
Formula Weight
301.25
MOL File
369-07-3.mol
More
Less

2-Nitrophenyl-beta-D-galactopyranoside Property

Melting point:
186-193 °C (dec.)
alpha 
-70 º (c=1, H2O)
Boiling point:
442.39°C (rough estimate)
Density 
1.3709 (rough estimate)
refractive index 
-68.8 ° (C=1, H2O)
storage temp. 
2-8°C
solubility 
DMSO (Slightly), Water (Slightly, Heated, Sonicated)
pka
12.69±0.70(Predicted)
form 
Powder
color 
White to very slightly yellow
optical activity
[α]20/D 70±1°, c = 1% in H2O
Water Solubility 
Soluble in water.
BRN 
92207
InChIKey
KUWPCJHYPSUOFW-RMPHRYRLSA-N
CAS DataBase Reference
369-07-3(CAS DataBase Reference)
NIST Chemistry Reference
Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)
More
Less

Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
F 
3-10-21
HS Code 
29400090
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
1g
Price
$54.7
Updated
2024/03/01
Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
5G
Price
$208
Updated
2024/03/01
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
1G
Price
$40.3
Updated
2022/05/15
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
5G
Price
$142
Updated
2022/05/15
TCI Chemical
Product number
N0418
Product name
2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
Purity
>98.0%(HPLC)
Packaging
1g
Price
$30
Updated
2021/12/16
More
Less

2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Usage,Production

Description

2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.

Chemical Properties

White Crystalline Solid

Uses

2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.

Definition

ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.

General Description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Purification Methods

Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]

2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

2-Nitrophenyl-beta-D-galactopyranoside Suppliers

Austria 1 Belgium 1 Canada 3 China 340 Czech Republic 1 Europe 3 France 1 Germany 8 Hungary 1 India 22 Italy 1 Japan 5 Poland 1 Russia 1 Slovakia 1 Switzerland 8 The Netherlands 1 United Kingdom 21 United States 43 Global 463
Hunan Waybel Advanced Materials Co.,Ltd
Tel
133-0845-3923 13319523173
Email
ivy@hnhbsj.com
Country
China
ProdList
457
Advantage
60
Hunan Hui Bai Shi Biotechnology Co., Ltd.
Tel
0731-85526065 13308475853
Email
ivy@hnhbsj.com
Country
China
ProdList
4554
Advantage
62
Nanjing UltraPure Biotech Co., Ltd.
Tel
025-82263158 13851993283
Fax
025-82263168
Email
2842979086@qq.com
Country
China
ProdList
241
Advantage
58
Nantong puyue biomedical Co., Ltd
Tel
0513-0513-0513-89158300 18014392889
Fax
0513-89158198
Email
sales@puyerbiopharma.com
Country
China
ProdList
4677
Advantage
58
Jinan Daen Pharmaceutical Technology Co., Ltd
Tel
0531-68657833 15553112864
Fax
-
Email
2098511197@qq.com
Country
China
ProdList
219
Advantage
58
Chongqing Xingcan Pharmaceutical Technology Co., Ltd.
Tel
15523000485
Email
xingcanyaoye@sina.com
Country
China
ProdList
1687
Advantage
58
Jinan Kabotang Biological Technology Co.,Ltd.
Tel
0531-61320525 15866703830
Email
495745175@qq.com
Country
China
ProdList
6897
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
future industrial shanghai co., ltd
Tel
400-0066400 13621662912
Fax
021-55660885
Email
sales@jonln.com
Country
China
ProdList
1995
Advantage
65
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44688
Advantage
61
Feiyang Biotechnology Co., Ltd
Tel
0533-7866336 13573361699
Fax
+86-533-7866337
Email
sales@chemfy.com
Country
China
ProdList
524
Advantage
62
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
Chemsky（shanghai）International Co.,Ltd.
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
32321
Advantage
50
China DongFan Chemical Co.,LTD
Tel
86-0571-85151182
Fax
86-0571-85151182
Country
China
ProdList
5691
Advantage
66
Qingdao Vochem Co., Ltd.
Tel
532-88066095 18661779210
Email
vochem@vochem.com
Country
China
ProdList
98
Advantage
60
Beijing Isomersyn Technology CO;LTD
Tel
010-82954736 13391601435
Email
sales@isomersyn.com
Country
China
ProdList
3295
Advantage
57
Shandong Xiya Chemical Co., Ltd
Tel
4009903999 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18738
Advantage
57
Syntechem Co.,Ltd
Tel
Fax
E-Mail Inquiry
Email
info@syntechem.com
Country
China
ProdList
12984
Advantage
57
Tangshan Moneide Trading Co., Ltd.
Tel
0315-8309571 15633399667
Fax
+86-315-7726572
Email
sales@moneidechem.com
Country
China
ProdList
704
Advantage
57
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11707
Advantage
57
RYSS TECH LTD
Tel
400-188-0725 +86 21 34310725 13611771617
Fax
+86 21 34311076
Country
China
ProdList
2002
Advantage
57
Sinopharm Chemical Reagent Co,Ltd.
Tel
86-21-63210123
Fax
86-21-63290778 86-21-63218885
Email
sj_scrc@sinopharm.com
Country
China
ProdList
9815
Advantage
79
Hangzhou Yuhao Chemical Technology Co., Ltd
Tel
0571-82693216
Fax
+86-571-82880190
Email
info@yuhaochemical.com
Country
China
ProdList
9387
Advantage
52
ZhengZhou HuaWen Chemical Co.Ltd
Tel
0370-2785118 17550508557
Fax
QQ：3470079902
Email
675141927@qq.com
Country
China
ProdList
3203
Advantage
55
Spectrum Chemical Manufacturing Corp.
Tel
021-021-021-67601398-809-809-809 15221380277
Fax
021-57711696
Email
marketing_china@spectrumchemical.com
Country
China
ProdList
9658
Advantage
60
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
meilunui@163.com
Country
China
ProdList
4727
Advantage
58
Nanjing Asian Chemical Co., Ltd.
Tel
+86 (25) 82263158
Fax
+86 (25) 82263168
Country
China
ProdList
262
Advantage
60
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4940
Advantage
60
T&W GROUP
Tel
021-61551611 13296011611
Fax
＋86 21-50676805
Email
contact@trustwe.com
Country
China
ProdList
9895
Advantage
58
Shanghai civi chemical technology co.,Ltd
Tel
86-21-34053660
Fax
86-21-34053661
Email
sale@labgogo.com
Country
China
ProdList
9865
Advantage
52
Thermo Fisher Scientific
Tel
800-810-5118
Fax
+86-10-84193589
Email
cnchemical@thermofisher.com
Country
China
ProdList
17770
Advantage
75
Beijing HuaMeiHuLiBiological Chemical
Tel
010-56205725
Fax
010-65763397
Email
waley188@sohu.com
Country
China
ProdList
12335
Advantage
58
NCE Biomedical Co.,Ltd.
Tel
4000-027-021 |24 +86-13986109188 | +86-15623472865 | +81-08033611988
Fax
+86-27-87599188
Country
China
ProdList
1493
Advantage
55
Haoyuan Chemexpress Co., Ltd.
Tel
021-58950125
Fax
(86) 21-58955996
Email
info@chemexpress.com
Country
China
ProdList
7552
Advantage
61
9ding chemical ( Shanghai) Limited
Tel
4009209199
Fax
86-021-52271987
Email
sales@9dingchem.com
Country
China
ProdList
22514
Advantage
55
Nanjing Baifuli Technology Co., Ltd.
Tel
25-25-58740604 15951658055
Fax
+86-25-58356858
Email
sales@unisyn.cn
Country
China
ProdList
561
Advantage
58
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-400-6206333 18521732826
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25003
Advantage
65
Shanghai Tauto Biotech Co., Ltd.
Tel
021-51320588
Fax
0086-21-51320502
Email
tauto@tautobiotech.com
Country
China
ProdList
3989
Advantage
66
Nanjing Dulai Biotechnology Co., Ltd.
Tel
025-84699383-8003 18013301590
Fax
025-84699383-8003
Email
njduly@126.com
Country
China
ProdList
971
Advantage
55
Wuxi Zhangkun Biochemical Technology Co., Ltd.
Tel
+86 0510-85629785
Fax
+86 0510-85625359
Country
China
ProdList
2327
Advantage
58
TargetMol Chemicals Inc.
Tel
021-021-33632979 15002134094
Fax
021-33632979
Email
marketing@targetmol.com
Country
China
ProdList
7783
Advantage
58
Shanghai BeiZhuo Biotech Co., Ltd.
Tel
021-61119791,13386096464
Fax
021-50190009
Email
bzswkf@foxmail.com
Country
China
ProdList
3921
Advantage
50
Vientiane Tianjin Hengyuan Technology Co., Ltd.
Tel
15722085254
Fax
022-26358246
Email
phytochemical@126.com
Country
China
ProdList
813
Advantage
55
Bide Pharmatech Ltd.
Tel
400-164-7117 13681763483
Fax
+86-21-61629029
Email
product02@bidepharm.com
Country
China
ProdList
41462
Advantage
60
More
Less

View Lastest Price from 2-Nitrophenyl-beta-D-galactopyranoside manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $0.00/KG
Min. Order
1KG
Purity
98.0%
Supply Ability
500kg/month
Release date
2023-01-09
Hebei Weibang Biotechnology Co., Ltd
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-11-05
Hebei Yanxi Chemical Co., Ltd.
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $40.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10 tons
Release date
2023-09-25

369-07-3, 2-Nitrophenyl-beta-D-galactopyranosideRelated Search:

2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE 2-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE Sodium 3-nitrobenzenesulphonate 4-Nitrobenzoic acid 4-Nitrobenzaldehyde α-galactosidase DIGITONIN Nitrobenzene 4-Nitrophenyl-beta-D-galactopyranoside 1,3-Dinitrobenzene 4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE, 98+%,4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE HYDRATE,4-Nitrophenyl beta-D-galactopyranoside 2-hexadecanoylamino-4-nitrophenyl-beta-D-galactopyranoside 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE-6-PHOSPHATE DICYCLOHEXYL P-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE TETRAACETATE O-NITROPHENYL BETA-D-GALACTOPYRANOSIDE-6-PHOSPHATE, CYCLOHEXYLAMMONIUM SALT m-nitrophenyl beta-D-galactopyranoside ,3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE 2-CHLORO-4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE ρ-Nitrobenzenesulphonic acid Onpg Solution

  • 2-Nitrophenyl hexopyranoside
  • 2-Nitrophenyl-beta-delta-galactopyranoside
  • 2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
  • o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
  • beta-D-Galactopyranoside, 2-nitrophenyl
  • beta-d-galactopyranoside,2-nitrophenyl
  • Galactopyranoside, o-nitrophenyl, beta-D-
  • o-Nitrophenyl beta-galactoside
  • 2-Nitrophenyl-β-D-galactopyranoside
  • ONPG o-Nitrophenyl-?-D-galactoside
  • 2'-Nitrophenyl-D-galactopyranoside (ONPG)
  • 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE, 99+%
  • 2-Nitrophenyl-B-D-Galactopyranoside(Onpg)
  • 2-Nitrophenyl-Beta-D-Galactopyranside
  • O-Nitrophenyl-Beta-D-GalactopyranosideA.R.
  • o-NPG=ONPG
  • 2-Nitrophenyl
  • Nitrophenyl-b-D-galactopyranoside
  • 2-nitrophenyl-á-d-galactopyranoside
  • ONPG（2-Nitrophenyl-beta-D-galactopyranoside
  • 2-Nitrophenyl b-D-galactoside-(1,5), ONPG
  • 2-Nitrophenyl-β-D-galactoside
  • 2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]
  • ortho-Nitrophenyl-β-galactoside
  • (2R,3R,4S,5R,6S)-2-(hydroxyMethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
  • Ortho nitroalpha -D-glucopyranoside
  • The 2- nitrophenyl beta-D- galactopyranoside
  • ortho-nitrophenyl β-D-galactopyranoside
  • 2-nitrophenyl β-d-[1-13C]galacto-pyranoside
  • 2-nitrophenyl β-d-[2-13C]galacto-pyranoside
  • TOLUIDINE BLUE O HIGH PURITY GRADE
  • 2-Nitrophenyl--D-galactopyranoside for synthesis
  • 2-Nitrophenyl-β-D-Galactopyranoside (plant origin)
  • 2-NITROPHENYL-B-D-GALACTOPYRANOSIDE
  • 2-NITROPHENYL-B-D-GALACTOSIDE
  • 2'-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE
  • 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE
  • 2-NITROPHENYL-BETA-D-GALACTOSIDE
  • 2-NITROPHENYL BETA-D-GALACTOSIDE-(1,5)
  • 2-NITROPHENYL-BETA-D-GALAKTOPYRANOSIDE
  • 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE (ONPG)
  • 2''-NITROPHENYL-SS-D-GALACTOPYRANOSIDE (ONPG)
  • 2-Nitrophenyl-β-D-galactopyranoside, 98+%
  • 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
  • o-NITROPHENYL-b-D-GALACTOPYRANOSIDE extrapure
  • o-Nitrophenyl β-D-galactopyranoside, ONPG
  • (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
  • O-Nitrophenyl-beta-d-galactopyranoside 99%
  • 2-Nitrophenyl-^b-D-galactopyranoside, 98+%
  • 2-Nitrophenyl-b-D-galactopy
  • 2-Nitrophenyl-b-D-glucosidase
  • 2-Nitrophenyl-beta-D-galactopyranoside, 98+%
  • 2-Nitrophenyl β-D-galactopyranoside,o-Nitrophenyl β-D-galactopyranoside, ONPG
  • 2-Nitrophenyl-beta-D
  • 2-NITROPHENYL-SS-D-GALACTOPYRANOSIDE
  • 2-Nitrophenyl b-D-galactopyranoside - non-animal origin
  • ONPG
  • ONP-BETA-D-GAL