Phenosafranin

Product Name: Phenosafranin
CAS No.: 81-93-6
Chemical Name: Phenosafranin
Synonyms: nsc9855;CI 50200;CI NO 50200;schultzno.958;PHENOSAFRANIN;phenosofranine;Phenosafeanine;phenosofrinine;safrininbextra;PHENOSAFRANINE
CBNumber: CB1460102
Molecular Formula: C18H15ClN4
Formula Weight: 322.8
MOL File: 81-93-6.mol

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Phenosafranin Property

Melting point:: 300 °C
Boiling point:: 478.49°C (rough estimate)
Density: 1.1738 (rough estimate)
refractive index: 1.6110 (estimate)
storage temp.: room temp
Colour Index: 50200
form: Solid
color: Green to dark green
Merck: 13,7333
BRN: 3642082
CAS DataBase Reference: 81-93-6

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26-36
WGK Germany: 3
RTECS: SG1630000

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 199648
Product name: Phenosafranin
Purity: Dye content 80 %
Packaging: 1g
Price: $69.8
Updated: 2024/03/01

TRC

Product number: P318020
Product name: Phenosafranin
Packaging: 50mg
Price: $155
Updated: 2021/12/16

Usbiological

Product number: 241218
Product name: Phenosafranin
Packaging: 1g
Price: $156
Updated: 2021/12/16

Usbiological

Product number: 286776
Product name: Phenosafranin
Packaging: 25mg
Price: $446
Updated: 2021/12/16

Chem-Impex

Product number: 22902
Product name: Phenosafranin ,≥80%(Dyecontent)
Purity: ≥80%(Dyecontent)
Packaging: 1G
Price: $17.47
Updated: 2021/12/16

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Phenosafranin Chemical Properties,Usage,Production

Chemical Properties

green crystalline powder

Uses

Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.

Definition

ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium.

Biochem/physiol Actions

Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.

Staining Procedures

Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.

Purification Methods

Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]

Phenosafranin Preparation Products And Raw materials

Raw materials

Preparation Products

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Phenosafranin Suppliers

Belgium 1 Canada 2 China 81 Germany 2 India 7 Japan 1 Switzerland 1 United Kingdom 3 United States 18 Global 116

J & K SCIENTIFIC LTD.

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Adamas Reagent, Ltd.

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Chemsky (shanghai) International Co.,Ltd

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Sichuan Kulinan Technology Co., Ltd

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Tianjin heowns Biochemical Technology Co., Ltd.

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Sinopharm Chemical Reagent Co,Ltd.

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Spectrum Chemical Manufacturing Corp.

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Chengdu Ai Keda Chemical Technology Co., Ltd.

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9ding chemical ( Shanghai) Limited

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81-93-6, PhenosafraninRelated Search:

Janus Green B INDOINE BLUE 3-amino-7-(dimethylamino)-5-phenylphenazinium chloride CI NO 50215 RHODULINE RED AMETHYST VIOLET CI 50206 JANUS BLACK ACID BLUE 20 Basic Red 2 Phenosafranin

3,7-DIAMINO-5-PHENYLPHENAZINIUM CHLORIDE

CI 50200

CI NO 50200

3,7-diamino-5-phenyl-phenaziniuchloride

nsc9855

Phenazinium,3,7-diamino-5-phenyl-,chloride

phenosafranine,chloride

2,8-DiaMino-10-phenyl-phenaziniuM Chloride

Phenosafeanine

Phenazinium,2,8-diamino-10-phenyl-, chloride (1:1)

phenosofranine

phenosofrinine

safrininbextra

schultzno.958

PHENOSAFRANIN

PHENOSAFRANINE

PHENOSAFRANINE FOR MICROSCOPY

Safranine B Extra

3,7-Diamino-5-phenylphenazin-5-ium chloride

Phenosafranine, indicator

PHENOSAFFRANINE

81-93-6

C18H15ClN4

C18H15N4Cl

Amines

Aromatics

Mutagenesis Research Chemicals