Phenosafranin Property

Melting point:

300 °C

Boiling point:

478.49°C (rough estimate)

Density 

1.1738 (rough estimate)

refractive index 

1.6110 (estimate)

storage temp. 

room temp

Colour Index 

50200

form 

Solid

color 

Green to dark green

Merck 

13,7333

BRN 

3642082

CAS DataBase Reference

81-93-6

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

SG1630000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Phenosafranin Chemical Properties,Usage,Production

Chemical Properties

green crystalline powder

Uses

Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.

Definition

ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium.

Biochem/physiol Actions

Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.

Staining Procedures

Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.

Purification Methods

Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]