ChemicalBook > CAS DataBase List > Phenosafranin
Phenosafranin
- Product Name
- Phenosafranin
- CAS No.
- 81-93-6
- Chemical Name
- Phenosafranin
- Synonyms
- nsc9855;CI 50200;CI NO 50200;schultzno.958;PHENOSAFRANIN;phenosofranine;Phenosafeanine;phenosofrinine;safrininbextra;PHENOSAFRANINE
- CBNumber
- CB1460102
- Molecular Formula
- C18H15ClN4
- Formula Weight
- 322.8
- MOL File
- 81-93-6.mol
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Phenosafranin Property
- Melting point:
- 300 °C
- Boiling point:
- 478.49°C (rough estimate)
- Density
- 1.1738 (rough estimate)
- refractive index
- 1.6110 (estimate)
- storage temp.
- room temp
- Colour Index
- 50200
- form
- Solid
- color
- Green to dark green
- λmax
- 519 nm
- Merck
- 13,7333
- BRN
- 3642082
- CAS DataBase Reference
- 81-93-6
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Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
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N-Bromosuccinimide Price
Sigma-Aldrich
- Product number
- 199648
- Product name
- Phenosafranin
- Purity
- Dye content 80 %
- Packaging
- 1g
- Price
- $72.3
- Updated
- 2025/07/31
TRC
- Product number
- P318020
- Product name
- Phenosafranin
- Packaging
- 50mg
- Price
- $155
- Updated
- 2021/12/16
Usbiological
- Product number
- 241218
- Product name
- Phenosafranin
- Packaging
- 1g
- Price
- $156
- Updated
- 2021/12/16
Usbiological
- Product number
- 286776
- Product name
- Phenosafranin
- Packaging
- 25mg
- Price
- $446
- Updated
- 2021/12/16
Chem-Impex
- Product number
- 22902
- Product name
- Phenosafranin ,≥80%(Dyecontent)
- Purity
- ≥80%(Dyecontent)
- Packaging
- 1G
- Price
- $17.47
- Updated
- 2021/12/16
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Phenosafranin Chemical Properties,Usage,Production
Chemical Properties
green crystalline powder
Uses
Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.
Definition
ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium.
Biochem/physiol Actions
Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.
Staining Procedures
Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.
Purification Methods
Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]
Phenosafranin Preparation Products And Raw materials
Raw materials
Preparation Products
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81-93-6, PhenosafraninRelated Search:
Janus Green B
INDOINE BLUE
3-amino-7-(dimethylamino)-5-phenylphenazinium chloride
CI NO 50215
RHODULINE RED
AMETHYST VIOLET
CI 50206
JANUS BLACK
ACID BLUE 20
Basic Red 2
Phenosafranin