ChemicalBook > CAS DataBase List > 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Product Name: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
CAS No.: 207738-08-7
Chemical Name: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
Synonyms: abs9123;TMB-2HCL-2HO;TMB*2HCL*2H2O;TMB 2HC1.xH2O;TMB 2HCl.xH2O;TMB2HC1 hydrate;TMB Hydrochloride;TMB (hydrochloride hydrate);TMB (dihydrochloride x.hydrate);5'-Tetramethylbenzidine 2HCl 2H20
CBNumber: CB1500794
Molecular Formula: C16H23ClN2O
Formula Weight: 294.82
MOL File: 207738-08-7.mol

Less

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Property

Melting point:: >300°C
storage temp.: 2-8°C
solubility: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
form: Powder
color: Light brown to brown
Sensitive: Moisture & Light Sensitive
InChI: InChI=1S/C16H20N2.ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;1H;1H2
InChIKey: NYNRGZULARUZCC-UHFFFAOYSA-N
SMILES: CC1=C(C(C)=CC(C2C=C(C)C(N)=C(C)C=2)=C1)N.Cl.O
CAS DataBase Reference: 207738-08-7

Less

Safety

Risk Statements: 22
Safety Statements: 22-24/25-25-24
WGK Germany: 3
TSCA: Yes
HS Code: 29215900

Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

Less

N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 87750
Product name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity: ≥98.0% (calc. on dry substance, AT)
Packaging: 1g
Price: $91.5
Updated: 2025/07/31

Sigma-Aldrich

Product number: 861510
Product name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity: 97%
Packaging: 1g
Price: $116
Updated: 2025/07/31

Sigma-Aldrich

Product number: T8768
Product name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity: powder, ≥98%
Packaging: 100mg
Price: $82.8
Updated: 2025/07/31

Sigma-Aldrich

Product number: T8768
Product name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity: powder, ≥98%
Packaging: 1g
Price: $129
Updated: 2025/07/31

Sigma-Aldrich

Product number: T8768
Product name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity: powder, ≥98%
Packaging: 5g
Price: $440
Updated: 2025/07/31

Less

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Chemical Properties,Usage,Production

Description

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λ_max = 652 nm) or 1 mole of diimine (λ_max = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

Chemical Properties

Off-white fluffy powder

Uses

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.

Uses

Convenient water-soluble form of TMB for determination of peroxidase.

Uses

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

General Description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

References

[1] PANAKKEZHUM D. THOMAS Mark J P. A modified tetramethylbenzidine method for measuring lipid hydroperoxides[J]. Analytical biochemistry, 1990, 188 1: Pages 228-232. DOI: 10.1016/0003-2697(90)90557-p
[2] LEAH A. MARQUEZ H. B D. Mechanism of the Oxidation of 3,5,3‘,5‘-Tetramethylbenzidine by Myeloperoxidase Determined by Transient- and Steady-State Kinetics?[J]. Biochemistry Biochemistry, 1997, 36 31: 9349-9355. DOI: 10.1021/bi970595j
[3] P. JOSEPHY R M T Eling. The horseradish peroxidase-catalyzed oxidation of 3,5,3’,5’-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.[J]. The Journal of Biological Chemistry, 1982, 31 1: 3669-3675. DOI: 10.1016/s0021-9258(18)34832-4
[4] S. MADERSBACHER P. B. Double wavelength measurement of 3,3′,5,5′-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range[J]. Journal of immunological methods, 1991, 138 1: Pages 121-124. DOI: 10.1016/0022-1759(91)90071-m
[5] ARMANDO REYNA-BELLO Adriana M S Mariana C Eleizalde. Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosis.[J]. Journal of immunoassay & immunochemistry, 2007, 28 1: 1-11. DOI: 10.1080/15321810601025432

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Preparation Products And Raw materials

Raw materials

Preparation Products

Less

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Suppliers

Belgium 1 China 179 France 1 Germany 1 India 4 South Korea 1 Switzerland 2 United Kingdom 1 United States 4 Global 194

Jiangsu Hi-Pure Technology New Materials Co. Ltd.

Tel: 0512-62764072; 18051857768
Email: sales@hi-pure.cn
Country: China
ProdList: 38
Advantage: 58

Hunan Tengyu New Materials Co., Ltd.

Tel: 1822-9738810 18229738810
Email: 3590064534@qq.com
Country: China
ProdList: 146
Advantage: 58

J & K SCIENTIFIC LTD.

Tel: 18210857532; 18210857532
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
Country: China
ProdList: 96815
Advantage: 76

Meryer (Shanghai) Chemical Technology Co., Ltd.

Tel: 4006356688 18621169109
Fax: 86-21-61259102
Email: market03@meryer.com
Country: China
ProdList: 40228
Advantage: 62

Energy Chemical

Tel: 021-021-58432009 400-005-6266
Fax: 021-58436166
Email: sales8178@energy-chemical.com
Country: China
ProdList: 44801
Advantage: 61

Nanjing Chemlin Chemical Co., Ltd

Tel: 025-83697070
Fax: +86-25-83453306
Email: info@chemlin.com.cn
Country: China
ProdList: 15883
Advantage: 64

Shanghai Hanhong Scientific Co.,Ltd.

Tel: 021-54306202 13764082696
Email: info@hanhongsci.com
Country: China
ProdList: 42934
Advantage: 64

XiaoGan ShenYuan ChemPharm co,ltd

Tel: 15527768836
Email: 1791901229@qq.com
Country: China
ProdList: 6950
Advantage: 52

Tangshan Moneide Trading Co., Ltd.

Tel: 0315-8309571 15633399667
Fax: +86-315-7726572
Email: sales@moneidechem.com
Country: China
ProdList: 704
Advantage: 57

Tianjin heowns Biochemical Technology Co., Ltd.

Tel: 400 638 7771
Email: sales@heowns.com
Country: China
ProdList: 14435
Advantage: 57

Sinopharm Chemical Reagent Co,Ltd.

Tel: 86-21-63210123
Fax: 86-21-63290778 86-21-63218885
Email: sj_scrc@sinopharm.com
Country: China
ProdList: 9815
Advantage: 79

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Tel: 0731-85526065 13308475853
Email: ivy@hnhbsj.com
Country: China
ProdList: 7247
Advantage: 62

ShangHai YuanYe Biotechnology Co., Ltd.

Tel: 021-61312847 13636370518
Fax: 021-55068248
Email: shyysw007@163.com
Country: China
ProdList: 4999
Advantage: 60

Thermo Fisher Scientific

Tel: 800-810-5118
Fax: +86-10-84193589
Email: cnchemical@thermofisher.com
Country: China
ProdList: 17770
Advantage: 75

Shanghai Aladdin Bio-Chem Technology Co.,LTD

Tel: 400-6206333 13167063860
Fax: 021-50323701
Email: anhua.mao@aladdin-e.com
Country: China
ProdList: 25003
Advantage: 65

China Nobel Chem Co., Limited

Tel: +86(0)21 60484900
Fax: +86(0)21 60484900
Country: China
ProdList: 764
Advantage: 50

Bide Pharmatech Ltd.

Tel: 400-164-7117 13681763483
Fax: +86-21-61629029
Email: product02@bidepharm.com
Country: China
ProdList: 39966
Advantage: 60

Suzhou Laing Biological Technology Co., LTD

Tel: 0512-62968256 18550502935
Fax: 0512-62968256
Email: export@laingbiotec.com
Country: China
ProdList: 976
Advantage: 58

Suzhou yacoo science co.,Ltd

Tel: 0512-87182056 18013166090
Fax: 0512-87182056
Email: lingling.qi@yacoo.com.cn
Country: China
ProdList: 6295
Advantage: 60

Shanghai Macklin Biochemical Co.,Ltd.

Tel: 15221275939
Fax: 021-50706099
Email: shenlinxing@macklin.cn
Country: China
ProdList: 15873
Advantage: 55

Hangzhou J&H Chemical Co., Ltd.

Tel: 0571-87396432
Fax: 0571-87396431
Email: sales@jhechem.com
Country: China
ProdList: 14912
Advantage: 59

Jiangsu Aikon Biopharmaceutical R&D co.,Ltd.

Tel: 025-66028182 18626450290
Email: yftan@aikonchem.com
Country: China
ProdList: 16679
Advantage: 50

Suzhou Haiheng Biological Pharmaceutical Co., Ltd.

Tel: 0512-65045967 15365387483
Fax: 15365387483
Email: sales@haihengpharm.com
Country: China
ProdList: 95
Advantage: 55

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51395
Advantage: 80

Chengdu RunZeBenTu Chemical Co., Ltd

Tel: 028-88469284 18000562381
Email: rzbtsj@163.com
Country: China
ProdList: 9951
Advantage: 56

Shanghai Haozhixiang Biotechnology Co., Ltd

Tel: 526763801 13301653310
Fax: +86-21-51619052
Email: 526763801@qq.com
Country: China
ProdList: 9274
Advantage: 55

Shandong Xiya Chemical Co., Ltd.

Tel: 4009903999 13395398332
Fax: 0539-6365991
Email: sales@xiyashiji.com
Country: China
ProdList: 20794
Advantage: 60

Grader reagent

Tel: 18221735425
Email: sales@xinpingchem.com
Country: China
ProdList: 9942
Advantage: 58

Guangdong wengjiang Chemical Reagent Co., Ltd.

Tel: 0751-2886750 13927877953
Fax: 0751-2886750
Email: 3005811397@qq.com
Country: China
ProdList: 13305
Advantage: 58

Beijing Jin Ming Biotechnology Co., Ltd.

Tel: 010-60605840 18892239720
Fax: 010-60605840
Email: psaitong@jm-bio.com
Country: China
ProdList: 12306
Advantage: 58

Shanghai Yihe Biological Technology Co., Ltd.

Tel: 021-68882955 17721395025
Fax: 021-68882955
Email: 2423903095@qq.com
Country: China
ProdList: 4991
Advantage: 58

Zhengzhou Huiju Chemical Co., Ltd.

Tel: 0371-55900031 18137872243
Fax: QQ：2853979815
Email: 2853979815@qq.com
Country: China
ProdList: 9975
Advantage: 55

Henan Anky Chemical Technology Co., Ltd.

Tel: 17836913271
Fax: 0374-8348899
Email: 3224305243@qq.com
Country: China
ProdList: 2944
Advantage: 58

Hunan Waybel Advanced Materials Co.,Ltd

Tel: 0731-85529965 13319523173
Email: 3002708854@qq.com
Country: China
ProdList: 481
Advantage: 60

Shanghai BaiShun Biological Technology Co., Ltd

Tel: 021-37581181
Fax: 021-57456066
Email: sales@chem-mall.com
Country: China
ProdList: 10330
Advantage: 58

Hubei XinghengKang Chemical Technology Co., Ltd.

Tel: 027-88878869 18162384755
Fax: 027-88878869
Email: xhkhu2014@163.com
Country: China
ProdList: 240
Advantage: 58

Shanghai Zhen Li Biological Technology Co., Ltd

Tel: 021-16621358918; 18516310516
Fax: 13045646768
Email: zhenlipharma888@163.com
Country: China
ProdList: 19035
Advantage: 58

Rhawn Reagent

Tel: 400-400-1332688 18019345275
Fax: 400-133-2688
Email: amy@rhawn.cn
Country: China
ProdList: 14948
Advantage: 58

Shanghai Haohong Pharmaceutical Co., Ltd.

Tel: 400-8210725 400821072
Fax: 021-58955996
Email: malulu@leyan.com
Country: China
ProdList: 25000
Advantage: 58

Twochem Co.Ltd.

Tel: 021-58111628 15800915896
Fax: QQ 3285589261
Email: sales@twochem.com
Country: China
ProdList: 1788
Advantage: 58

Tianjin heowns Biochemical Technology Co., Ltd.

Tel: 400-6387771-6039 18920764717
Email: sales@heowns.com
Country: China
ProdList: 23771
Advantage: 60

Hubei Xinyuanshun Pharmaceutical Chemical Co., Ltd.

Tel: 027-51831887 15337167856
Fax: 027-51837635 QQ1165326703
Email: hubeixinyuanshun@163.com
Country: China
ProdList: 10295
Advantage: 58

Shanghai neon biological technology co., LTD

Tel: 021-54252912-8903
Email: leona.xin@forneeds.cn
Country: China
ProdList: 4759
Advantage: 58

Zhengzhou Acme Chemical Co., Ltd.

Tel: 0371-037163312495,13303845143 13303845143
Fax: QQ3001379618
Email: 3001379618@qq.com
Country: China
ProdList: 10267
Advantage: 58

Shanghai Jizhi Biochemical Technology Co. Ltd.

Tel: 4009004166/18616739031 18616739031
Email: 3007523370@qq.com
Country: China
ProdList: 52687
Advantage: 58

Beijing OKA biological technology co., LTD

Tel: 010-62971590 18548936886
Fax: 010-62340519
Email: 3462612863@qq.com
Country: China
ProdList: 10063
Advantage: 58

Shanghai Hongye Biotechnology Co. Ltd

Tel: 400-9205774
Email: sales@glpbio.cn
Country: China
ProdList: 6777
Advantage: 58

Guangdong wengjiang Chemical Reagent Co., Ltd.

Tel: 0751-2815688 13927872512
Fax: 0751-8963795
Email: 3007421951@qq.com
Country: China
ProdList: 9939
Advantage: 58

Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.

Tel: 0755-23251735 13302967066
Email: sales@geneseqtools.com
Country: China
ProdList: 1420
Advantage: 58

Hefei Molake Biotechnology Co., Ltd.

Tel: 0551-66336380 18956014586
Fax: 055166336380
Email: 983438788@qq.com
Country: China
ProdList: 2297
Advantage: 58

Less

View Lastest Price from 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE manufacturers

Career Henan Chemical Co

Product: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+% 207738-08-7
Price: US $500.00/KG
Min. Order: 1KG
Purity: 98%
Supply Ability: 10tons
Release date: 2018-08-04

207738-08-7, 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATERelated Search:

4-AMINO-3-METHYLBIPHENYL 3,3',5,5'-Tetramethylbenzidine dihydrochloride 2,6-DIMETHYLANILINE HYDROCHLORIDE O-Tolidine 3'-METHYL-BIPHENYL-4-YLAMINE Tetramethylbenzidine 3,3',5,5'-TETRAMETHYLBIPHENYL 4-Aminobiphenyl hydrochloride Benzidine dihydrochloride 2,4,6-Trimethylaniline 3,3'-Dimethylbenzidine dihydrochloride 2,4-Dimethylaniline hydrochloride 3,3'-Dimethylbiphenyl 3,5-DIMETHYL-BIPHENYL 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

TMB2HC1 hydrate

TMB*2HCL*2H2O

3,3',5,5'-tetramethylbenzidine·2HCl·2H2O

3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate(1:2:x)

3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hydrate

TMB-2HCL-2HO

5'-Tetramethylbenzidine 2HCl 2H20

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%

3,3μ,5,5μ-Tetramethylbenzidine hydrate dihydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate,TMB dihydrochloride

TMB (hydrochloride hydrate)

3,3',5,5'-tetraMethylbiphenyl-4,4'-diaMine dihydrochloride hydrate (1:2:)

3,3',5,5'-TetraMethylbenzidine dihydrochloride hydrate 97%

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate Vetec(TM) reagent grade, >=98%

TMB 2HC1.xH2O

TMB 2HCl.xH2O

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x)

abs9123

3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride xhydrate

3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hyd...

TMB Hydrochloride

3,3,5,5-Tetramethyl Benzidine Dihydrochloride Hydrate (TMB.2HCl.xH2O) extrapure, 98%

4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline,hydrate,dihydrochloride

TMB 2HCl|3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

TMB (dihydrochloride x.hydrate)

3,3 ', 5,5' - Tetramethylbenzidine dihydrochloride salt water complex

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

3,3'',5,5''-Tetramethylbenzidine dihydrochloride hydrate

3,3'',5,5''-Tetramethylbenzidine dihydrochloride

3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine dihydrochloride dihydrate

5'-Tetramethylbenzidinedihydrochloridehydrate

3,3',5,5'-Tetramethyl-biphenyl-4,4'-diamine HCl hydrate

207738-08-7

-C6H2CH324NH222HClxH2O