ChemicalBook > CAS DataBase List > 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Product Name
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
CAS No.
207738-08-7
Chemical Name
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
Synonyms
abs9123;TMB-2HCL-2HO;TMB*2HCL*2H2O;TMB 2HC1.xH2O;TMB 2HCl.xH2O;TMB2HC1 hydrate;TMB Hydrochloride;TMB (hydrochloride hydrate);TMB (dihydrochloride x.hydrate);5'-Tetramethylbenzidine 2HCl 2H20
CBNumber
CB1500794
Molecular Formula
C16H23ClN2O
Formula Weight
294.82
MOL File
207738-08-7.mol
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3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Property

Melting point:
>300°C
storage temp. 
2-8°C
solubility 
Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
form 
Powder
Sensitive 
Moisture & Light Sensitive
InChIKey
NYNRGZULARUZCC-UHFFFAOYSA-N
CAS DataBase Reference
207738-08-7
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Safety

Risk Statements 
22
Safety Statements 
22-24/25-25-24
WGK Germany 
3
TSCA 
Yes
HS Code 
29215900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
87750
Product name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity
≥98.0% (calc. on dry substance, AT)
Packaging
1g
Price
$88.7
Updated
2024/03/01
Sigma-Aldrich
Product number
861510
Product name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity
97%
Packaging
1g
Price
$113
Updated
2024/03/01
Alfa Aesar
Product number
J60332
Product name
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, 98%
Packaging
1g
Price
$83.65
Updated
2024/03/01
Alfa Aesar
Product number
J60332
Product name
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, 98%
Packaging
2.5g
Price
$134.65
Updated
2024/03/01
Sigma-Aldrich
Product number
T8768
Product name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Purity
powder, ≥98%
Packaging
100mg
Price
$80.3
Updated
2024/03/01
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3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Chemical Properties,Usage,Production

Description

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

Chemical Properties

Off-white fluffy powder

Uses

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.

Uses

Convenient water-soluble form of TMB for determination of peroxidase.

Uses

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

General Description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE manufacturers

Wuhan Biocar Pharmacy Co., Ltd.
Product
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE 207738-08-7
Price
US $9.90-9.90/g
Min. Order
5g
Purity
99.99%HPLC.USP42——Powder、Oil、Pills、Capsules、Tablets,Customiz
Supply Ability
100kg
Release date
2022-10-20
Career Henan Chemical Co
Product
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+% 207738-08-7
Price
US $500.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
10tons
Release date
2018-08-04

207738-08-7, 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATERelated Search:


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