Tetramethylbenzidine
- Product Name
- Tetramethylbenzidine
- CAS No.
- 54827-17-7
- Chemical Name
- Tetramethylbenzidine
- Synonyms
- TMB;Tmbz;TMB SUBSTRATE;Tetramethylbenzidine (TMB);3,3,5,5-TETRAMETHYLBENZIDINE, STANDARD F OR GC;4-(4-Amino-3,5-dimethylphenyl)-2,6-dimethylaniline;TMB-E;TMB-B;TMB/H;TMB/M
- CBNumber
- CB6682185
- Molecular Formula
- C16H20N2
- Formula Weight
- 240.34
- MOL File
- 54827-17-7.mol
Tetramethylbenzidine Property
- Melting point:
- 168-171 °C(lit.)
- Boiling point:
- 100 °C
- Density
- 1
- bulk density
- 430kg/m3
- refractive index
- 1.5519 (estimate)
- storage temp.
- 2-8°C
- solubility
- Slightly soluble. <0.1 g/100 mL at 20°C.
- pka
- 4.49±0.10(Predicted)
- form
- tablet
- color
- Cream
- Water Solubility
- Slightly soluble. <0.1 g/100 mL at 20 ºC
- Sensitive
- Light Sensitive
- BRN
- 2808541
- Stability:
- Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
- CAS DataBase Reference
- 54827-17-7(CAS DataBase Reference)
- EPA Substance Registry System
- 3,3',5,5'-Tetramethylbenzidine (54827-17-7)
Safety
- Hazard Codes
- Xi,Xn
- Risk Statements
- 36/38-36/37/38-22-41
- Safety Statements
- 26-36-36/37-39
- RIDADR
- UN 2796 8/PG 2
- WGK Germany
- 3
- RTECS
- DV2300000
- F
- 8-10
- TSCA
- Yes
- HazardClass
- 6.1
- HS Code
- 29215990
- Hazardous Substances Data
- 54827-17-7(Hazardous Substances Data)
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H225Highly Flammable liquid and vapour
H302Harmful if swallowed
H312Harmful in contact with skin
H315Causes skin irritation
H319Causes serious eye irritation
H332Harmful if inhaled
H335May cause respiratory irritation
H370Causes damage to organs
- Precautionary statements
-
P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.
P260Do not breathe dust/fume/gas/mist/vapours/spray.
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405Store locked up.
N-Bromosuccinimide Price
- Product number
- T5525
- Product name
- 3,3′,5,5′-Tetramethylbenzidine
- Purity
- tablet, 1 mg substrate per tablet
- Packaging
- 50 tabs
- Price
- $212.52
- Updated
- 2025/07/31
- Product number
- T5525
- Product name
- 3,3′,5,5′-Tetramethylbenzidine
- Purity
- tablet, 1 mg substrate per tablet
- Packaging
- 100 tabs
- Price
- $363.13
- Updated
- 2025/07/31
- Product number
- T4444
- Product name
- 3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA
- Purity
- ready to use solution
- Packaging
- 100 mL
- Price
- $126
- Updated
- 2025/07/31
- Product number
- T4444
- Product name
- 3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA
- Purity
- ready to use solution
- Packaging
- 4 x 100 mL
- Price
- $379
- Updated
- 2025/07/31
- Product number
- 11484281001
- Product name
- BM Blue POD Substrate, soluble
- Purity
- 3,3′-5,5′-Tetramethylbenzidine (TMB)
- Packaging
- 100mL
- Price
- $149
- Updated
- 2025/07/31
Tetramethylbenzidine Chemical Properties,Usage,Production
Description
Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.
Chemical Properties
Tetramethylbenzidine is a white or light yellow solid, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide and other organic solvents. It forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.
Uses
3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.
Preparation
The synthesis of 3,3',5,5'-Tetramethylbenzidine involved using 2,6-dimethylaniline as the raw material. The process included activation, oxidative coupling, and purification, resulting in the production of pure material. The yield of 3,3,5,5-tetramethylbenzidine was 65%.
Reactions
TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.
Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.
General Description
3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)
Air & Water Reactions
Insoluble in water.
Reactivity Profile
Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.
Fire Hazard
Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.
Biochem/physiol Actions
3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in?situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Synthesis
24596-19-8
54827-17-7
87-62-7
4-Bromo-2,6-dimethylaniline (24.0 parts) was added with 3% palladium charcoal (50% paste; 2.0 parts), cetyltrimethylammonium bromide (4.0 parts), 32% sodium hydroxide solution (13.5 parts), sodium formate (6.8 parts), and water (60 parts), and boiled and stirred under reflux conditions for 3 hours. Subsequently, sodium formate (6.8 parts) was added again and boiling at reflux was continued for 3 hours. Next, the reaction mixture was kept boiling under reflux conditions for 23 hours. Upon completion of the reaction, the water was removed by distillation and 2.2 parts (18.2%) of 2,6-dimethylaniline was collected. The remaining reaction mixture was cooled to room temperature and filtered, and the residue was continuously extracted with methanol (60 parts) for 5 hours. After the extract was cooled, a colorless crystalline precipitate was filtered out and dried to give 7.7 parts (63.3%) of 3,3',5,5'-tetramethylbenzidine.
Carcinogenicity
The carcinogenic potential of Tetramethylbenzidine is uncertain due to conflicting evidence. It is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
DOI: 10.1177/26.2.24068
References
[1] Patent: US4022795, 1977, A
Tetramethylbenzidine Preparation Products And Raw materials
Raw materials
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View Lastest Price from Tetramethylbenzidine manufacturers
- Product
- 3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- ≥98.5%
- Supply Ability
- 100KG
- Release date
- 2025-10-27
- Product
- TMB 54827-17-7
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 20MT
- Release date
- 2025-08-21
- Product
- Tetramethylbenzidine 54827-17-7
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 10000KGS
- Release date
- 2025-07-05