ChemicalBook > CAS DataBase List > Thiazolyl Blue

Thiazolyl Blue

Product Name
Thiazolyl Blue
CAS No.
298-93-1
Chemical Name
Thiazolyl Blue
Synonyms
MTT;THIAZOLYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLETETRAZOLIUM;METHYLTHIAZOLETETRAZOLIUM (MTT);MTT - CAS 298-93-1 - Calbiochem;THIAZOYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLYLDIPHENYL-TETRAZOLIUM BROMIDE;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE;2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2h-tetrazoliubromide;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
CBNumber
CB3383104
Molecular Formula
C18H16BrN5S
Formula Weight
414.32
MOL File
298-93-1.mol
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Thiazolyl Blue Property

Melting point:
195 °C (dec.) (lit.)
Density 
1.4886 (estimate)
storage temp. 
2-8°C
solubility 
methanol: 5 mg/mL, clear
form 
powder
color 
Yellow to orange
Water Solubility 
Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing
Sensitive 
Light Sensitive
λmax
378 nm, 242 nm
BRN 
3825277
Stability:
Hygroscopic
Biological Applications
Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer
InChIKey
AZKSAVLVSZKNRD-UHFFFAOYSA-M
CAS DataBase Reference
298-93-1(CAS DataBase Reference)
EPA Substance Registry System
2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1)
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Safety

Hazard Codes 
Xi,T,Xn
Risk Statements 
46-68-36/37/38
Safety Statements 
22-24/25-53-45-36/37-26
WGK Germany 
3
RTECS 
XF8060000
F 
3-8-10
TSCA 
Yes
HS Code 
29341000
Hazardous Substances Data
298-93-1(Hazardous Substances Data)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P308+P313IF exposed or concerned: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
475989
Product name
MTT - CAS 298-93-1 - Calbiochem
Purity
Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.
Packaging
1g
Price
$107
Updated
2024/03/01
Sigma-Aldrich
Product number
475989
Product name
MTT - CAS 298-93-1 - Calbiochem
Purity
Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.
Packaging
10g
Price
$742
Updated
2024/03/01
TCI Chemical
Product number
M3297
Product name
MTT [for Biochemical Research]
Packaging
1G
Price
$71
Updated
2024/03/01
TCI Chemical
Product number
D0801
Product name
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide
Purity
>98.0%(T)
Packaging
1g
Price
$111
Updated
2024/03/01
TCI Chemical
Product number
D0801
Product name
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide
Purity
>98.0%(T)
Packaging
5g
Price
$387
Updated
2024/03/01
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Thiazolyl Blue Chemical Properties,Usage,Production

Chemical Properties

Thiazolyl Blue Tetrazolium Blue (MTT) is a water soluble tetrazolium salt yielding a yellowish solution when prepared in media or salt solutions lacking phenol red. Dissolved MTT is converted to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water insoluble formazan can be solubilized using isopropanol or other solvents and the dissolved material is measured spectrophotometrically yielding absorbance as a function of concentration of converted dye.

Uses

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Definition

ChEBI: Thiazolyl Blue is the bromide salt of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. It has a role as a dye and a colorimetric reagent. It contains a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium.

General Description

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. It is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells. As an electron acceptor, it is utilized for studying nicotinamide adenine dinucleotide phosphate (NADP)-dependent dehydrogenases.

Biochem/physiol Actions

Cell permeable: yes

storage

+4°C

Purification Methods

It is recrystallised by dissolving in MeOH containing a few drops of HBr and then adding dry Et2O to complete the crystallisation, wash the needles with Et2O and dry them in a vacuum desiccator over KOH. [Beyer & Pyl Chem Ber 87 1505 1954, Beilstein 27 III/IV 6045.]

Thiazolyl Blue Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Thiazolyl Blue manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Thiazolyl Blue 298-93-1
Price
US $0.00/G
Min. Order
100G
Purity
98%min
Supply Ability
30kg/month
Release date
2023-02-06
Honest Joy Holdings Limited
Product
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide 298-93-1
Price
US $0.00/KG
Min. Order
1KG
Purity
99.3%
Supply Ability
100 tons
Release date
2022-02-20
Zhuozhou Wenxi import and Export Co., Ltd
Product
Thiazolyl Blue 298-93-1
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

298-93-1, Thiazolyl BlueRelated Search:

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  • 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazol-3-ium Bromide
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  • 3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHYL-2 H-TETRAZOLIUM BROMIDE
  • 3,[4,5-DIMETHYLTHIAZOL-2-YL]-2,5-DIPHENYLTETRAZOLIUM BROMIDE
  • 3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
  • 3-(4,5-DIMETHYLITHIAZOLYL-2)-2,5-DIPHENYL MONOTETRAZOLIUM BROMIDE
  • 3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE
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  • 2H-Tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-, bromide
  • 2,5-Diphenyl-3-(4,5-dimethyl-2-thiazolyl)-2H-tetrazole-3-ium·bromide
  • 2,5-Diphenyl-3-(4,5-dimethyl-2-thiazolyl)-3H-tetrazole-2-ium·bromide
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