5-FLUOROOROTIC ACID HYDRATE 98 Property

Melting point:

278 °C (dec.)(lit.)

storage temp. 

Inert atmosphere,Store in freezer, under -20°C

solubility 

Aqueous Base (Heated), DMSO (Sparingly, Heated), Methanol (Sparingly), Water (Sl

form 

Solid

color 

Must be White

Water Solubility 

Soluble in water, dimethyl sulfoxide, 50mg in 1ml (1:1 ammonium hydroxide: water) with gentle heating.

Merck 

14,4176

BRN 

181933

Safety

Hazard Codes 

Xn

Risk Statements 

22-36/37/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

UV7800000

HazardClass 

IRRITANT

HS Code 

2933599590

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

5-FLUOROOROTIC ACID HYDRATE 98 Chemical Properties,Usage,Production

Description

5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene, which encodes orotine-5''-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.

Uses

5-Fluoroorotic Acid, Monohydrate (5-FOA) has become a valuable tool for research in the field of molecular genetics and has been employed in the selection of resistant strains of Saccharomyces cerevisiae that possess a mutant URA3 gene which renders them orotidine-5í-phosphate decarboxylation.