ACES Property

Melting point:

>220 °C (dec.)(lit.)

Boiling point:

404.49℃

Density 

1.484±0.06 g/cm3(Predicted)

vapor pressure 

0Pa at 25℃

storage temp. 

room temp

solubility 

H₂O: 0.1 M at 20 °C, clear, colorless

form 

Crystals

color 

White

PH Range

6.1 - 7.5

PH

2.5-4.5 (25℃, 0.1M in H2O)

pka

6.8(at 25℃)

Water Solubility 

soluble

λmax

λ: 260 nm Amax: 0.008
λ: 280 nm Amax: 0.005

Merck 

14,36

BRN 

2253770

InChIKey

DBXNUXBLKRLWFA-UHFFFAOYSA-N

LogP

-4.49 at 25℃

CAS DataBase Reference

7365-82-4(CAS DataBase Reference)

EPA Substance Registry System

Ethanesulfonic acid, 2-[(2-amino-2-oxoethyl)amino]- (7365-82-4)

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

37/39-26-24/25

WGK Germany 

3

TSCA 

Yes

HS Code 

29241900

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P363Wash contaminated clothing before reuse.

P405Store locked up.

ACES Chemical Properties,Usage,Production

Description

ACES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is a paraacetamide buffer suitable for environments with a pH of 6.1 - 7.5. ACES can be used as a buffer component in cell culture media for use in agarose and polyacrylamide electrophoresis and in isoelectric focusing of proteins. It forms complexes with most common metals, and the formation constant should be taken into account when using such buffers in solutions containing metal ions.

Chemical Properties

ACES (N-(Carbamoylmethyl)taurine) is white crystals

Uses

ACES is used as a Good's buffer is an ideal separator in the resolution of protein systems, a zwitterionic compound used in biochemical and biological research.

Uses

N-(Carbamoylmethyl)taurine can be used to study biological buffers and zwitterionic compounds. ACES has been used in a study to determine the levels of ibuprofen and flurbiprofen in pharmaceuticals by capillary zone electrophoresis.

Uses

Buffer for biological systems.

Definition

ChEBI: ACES (N-(2-acetamido)-2-aminoethanesulfonic acid) is a Good's buffer substance, pKa = 6.9 at 20 ℃. It is an ACES and an amino sulfonic acid. It is a tautomer of a 2-[(2-amino-2-oxoethyl)ammonio]ethanesulfonate.

Preparation

In the presence of the reaction solvent tetrahydrofuran, add nitrogen-containing reagent ammonia into the reactor to react with the raw material, wherein the molar ratio of ammonia to theraw material is 1:1. The reaction temperature is 60°C, the reaction pressure is 1 MPa (gauge pressure), and the reaction time is 8 hours. After the reaction is completed, it is acidified to generate ACES. Then spin off the solvent tetrahydrofuran, add ethyl acetate, wash twice with water, dry with anhydrous sodium sulfate, and spin off the ethyl acetate to obtain the crude ACES. Purification stepIn the reactor, the purified solvent tetrahydrofuran was added to dissolve the crude ACES, recrystallized at low temperature, the insoluble matter was removed by suction, and the filtrate was rotary evaporated to remove tetrahydrofuran to obtain refined ACES.

Purification Methods

Recrystallise ACES from hot aqueous EtOH. [Perrin & Dempsey Buffers for pH and Metal Ion Control Chapman & Hall, London 1974, Beilstein 4 III 1707.]