CHAPS

Product Name: CHAPS
CAS No.: 75621-03-3
Chemical Name: CHAPS
Synonyms: CHAPS;TREND;3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE;N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[(3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl)amino]propylaminium;N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[[3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl]amino]-1-propaneaminium;1-Propanaminium, N,N-dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-, inner salt;CHAPS, 98+%;CHAPS buffer;CHAPS 100 mM;CHAPS, >=99%
CBNumber: CB4165631
Molecular Formula: C32H58N2O7S
Formula Weight: 614.88
MOL File: 75621-03-3.mol

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CHAPS Property

Melting point:: 156-158°C (dec.)
Density: 1.01 g/mL at 20 °C
refractive index: 1.6900 (estimate)
storage temp.: -20°C
solubility: H₂O: 50 mg/mL at 20 °C, clear, colorless
form: Crystalline Powder
color: White
Odor: Odorless
PH Range: 6
Water Solubility: Soluble in water (10 mg/ml). Soluble in DMF and hot methanol.
Sensitive: Hygroscopic
Merck: 2044
Stability:: Moisture Sensitive: Hydroscopic
InChIKey: UMCMPZBLKLEWAF-RFCNGIAKSA-N

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Safety

Hazard Codes: Xi,T
Risk Statements: 36/37/38-61
Safety Statements: 26-36-45-53
WGK Germany: 3
F: 3-10
HS Code: 29239000

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 220201
Product name: CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
Purity: CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
Packaging: 1g
Price: $81
Updated: 2024/03/01

Sigma-Aldrich

Product number: 220201
Product name: CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
Purity: CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
Packaging: 5G
Price: $239
Updated: 2024/03/01

Sigma-Aldrich

Product number: 220201
Product name: CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
Purity: CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
Packaging: 10g
Price: $398
Updated: 2024/03/01

Sigma-Aldrich

Product number: 220201
Product name: CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
Purity: CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
Packaging: 25g
Price: $786
Updated: 2024/03/01

Sigma-Aldrich

Product number: 220201
Product name: CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
Purity: CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
Packaging: 250g
Price: $2600
Updated: 2024/03/01

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CHAPS Chemical Properties,Usage,Production

Chemical Properties

White crystalline powder. CHAPS may be synthesized from cholic acid and is zwitterionic due to its quaternary ammonium and sulfonate groups; it is structurally similar to certain bile acids, such as taurodeoxycholic acid and taurochenodeoxycholic acid. It is sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity.

Uses

CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. It is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.

Application

CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.

General Description

CHAPS is a stabilizing agent that can be used to prevent aggregation, precipitation or proteolysis of proteins in solution.

Biological Activity

CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.

storage

Room temperature

Purification Methods

Triturate this zwitterionic detergent (~50g) with MeOH (200ml), then triturate it with Me2CO (500mL), collect it by vacuum filtration and dry it thoroughly at ~20o. Recrystallise it at 0o from absolute MeOH followed by drying in vacuo to constant weight. It should give one spot with RF 0.32 by TLC on silica gel G in 95% MeOH/5% NH4OH and visualized with iodine vapour; the tertiary amine precursor has RF 0.40. It has low UV absorption (1% in H2O: A280nm is 0.029 and A260nm is 0.035), and the CMC (critical micellar concentration) is 8mM. It is soluble in H2O, MeOH, Me2SO but insoluble in Me2CO, MeCN, hexane, toluene and xylene. [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate betaine) [82473-24-3] M 630.89, m 184-196o, [ ] D +19o (c 2, H2O), pKEst (1) ~<2 (acidic), pKEst(2) ~9 (basic). Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]

CHAPS Preparation Products And Raw materials

Raw materials

Preparation Products

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CHAPS Suppliers

Canada 3 China 329 Europe 4 France 1 Germany 11 India 25 Japan 4 Poland 1 South Korea 1 Switzerland 2 The Netherlands 1 United Kingdom 19 United States 63 Global 464

Toronto Research Chemicals

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View Lastest Price from CHAPS manufacturers

HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD

Product: CHAPS 75621-03-3
Price: US $100.00-75.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 5000
Release date: 2024-08-20

Apeloa production Co.,Limited

Product: CHAPS 75621-03-3
Price: US $1.00/g
Min. Order: 1g
Purity: 99%
Supply Ability: 2000
Release date: 2024-06-05

Hebei Yanxi Chemical Co., Ltd.

Product: 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate 75621-03-3
Price: US $0.00/kg
Min. Order: 1kg
Purity: 0.99
Supply Ability: 20 tons
Release date: 2023-11-21

75621-03-3, CHAPSRelated Search:

4-Dimethylaminobenzaldehyde Cocamidopropyl betaine 4-Amino-benzenesulfonic acid monosodium salt Sulfanilic acid N,N,N',N'-Tetramethylazodicarboxamide Sodium allylsulfonate Nickel bis(sulphamidate) Sulfamic acid 10-NONADECANOL N-OCTANAMIDE Cyclopentanemethanol 2,5-DIMETHYLCYCLOHEXANOL N-METHYL-N-OCTADECYLAMINE 1,16-HEXADECANEDIOL 2,6,8-TRIMETHYL-4-NONANOL (+/-)-NEOMENTHOL 7-AMINO-HEPTAN-1-OL Chapso

CHAPS

CHAPS (3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)PROPANESULFONATE)

3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANE SULPHONATE (ULTRAPURE)

3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]PROPANESULFONAT(CHAPS)

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulphonate (Ultrapure) >99.0%

CHAPS, 98+%

CHAPS extrapure

1-Propanaminium, N,N-dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-, inner salt

Cholane, 1-propanaminium deriv.

N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[(3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl)amino]propylaminium

N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[[3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl]amino]-1-propaneaminium

N-[3-(3-Sulfonatopropyldimethylaminio)propyl]-3α,7α,12α-trihydroxy-5β-cholan-24-amide

N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-1-propanaminium Inner Salt

3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfonate ,98%

3-(3-CHOLAMIDOPROPYL)DIMETHYL-AMMONIO-1-PROPANESULFONATE RESEARCH

CHAPS-SOL GRADE

3-((3-Cholamidopropy

3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate)

CHAPS buffer

CHAPS, Molecular Biology Grade

3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate

3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfote

CHAPS 100 mM

CHAPS, 3-[(3-Chloamidopropyl)dimethyl- ammonium]-1- propanesulfonate

3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE

3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANE SULPHONATE

3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)PROPANESULFONATE

3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate

3-[(3-Chloromidopropyl)dimethylammonio]-1-propanesulfonate

CHAPS 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

CHAPS Solution

CHAPS ELECTROPHORESIS REAGENT

Chaps3-[3-(Cholamidopropyl)Dimethylamonio]-1-Propanesulfonate

3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid

CHAPS,HighPurity

3-(3-(cholamidopropyl)dimethyl

Cholamidopropyl)dimethylammonio]-1-propanesulfonate

1-Propanaminium, N,N-dimethyl-N-(3-sulfopropyl)-3-(3.alpha.,5.beta.,7.alpha.,12.alpha.)-3,7,12-trihydroxy-24-oxocholan-24-ylamino-, inner salt

CHAPS,CRYSTALLINEPOWDER,ULTRAPURE

TREND

3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate (CHAPS)

3-[(3-cholaMidopropyl)diMethylaMMonio]propanesulfonate(CHAPS)

N,N-DiMethyl-N-(3-sulfopropyl)-3-[[(3α,5β,7α,12α)-3,7,12-trihydroxy- 24-oxocholan-24-yl]aMino]-1-propanaMiniuM Inner Salt

3-(Dimethyl(3-((4R)-4-((3R,5S,7R,10S,12S,13R,17R)-3,7,12-trihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanamido)propyl)ammonio)propane-1-sulfonate

CHAPS 100 mM solution

CHAPS Vetec(TM) reagent grade, 96%

CHAPS, >=99%

BRADFORD REAGENT BIOTECHNOLOGY GRADE

3-((3-((4R)-4-((10S,13R,17R)-10,13-DiMethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanaMido)propyl)diMethylaMMonio)propane-1-sulfonate

MEDIABAG ST FOR 3375 ML BPW

MEDIABAG ST FOR 4000 ML BPW

MEDIABAG ST FOR 450ML BPW 10 X10 ST

SMMP MEDIUM CONC.

TSA PETRI 9CM

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate &gt

3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate ISO 9001：2015 REACH

New reaserching product 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate CAS 75621-03-3 with best price