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BIS-Tris

Product Name
BIS-Tris
CAS No.
6976-37-0
Chemical Name
BIS-Tris
Synonyms
BIS-TRIS;BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE;2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol;Bis-Iris;2,2-BIS(HY;''BIS-TRIS'';BIS-TRIS >99%;BIS-TRIS >98%;BIS-TRIS BUFFER;BIS-Tris, ULTROL
CBNumber
CB4304881
Molecular Formula
C8H19NO5
Formula Weight
209.24
MOL File
6976-37-0.mol
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BIS-Tris Property

Melting point:
104 °C(lit.)
Boiling point:
466.2±40.0 °C(Predicted)
Density 
1.346±0.06 g/cm3(Predicted)
vapor pressure 
0Pa at 25℃
storage temp. 
Inert atmosphere,Room Temperature
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
Solution
color 
Yellow to orange
Odor
Odorless
PH Range
5.8 - 7.2
PH
9.0-10.1 (20-25℃, 1M in water)
pka
6.5(at 25℃)
Water Solubility 
soluble
Sensitive 
Air Sensitive
λmax
λ: 280 nm Amax: 0.15
BRN 
2205275
InChIKey
OWMVSZAMULFTJU-UHFFFAOYSA-N
LogP
-2.26 at 20℃
CAS DataBase Reference
6976-37-0(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/37/38-21/22-20/21/22
Safety Statements 
26-36-36/37/39-22
WGK Germany 
3
F 
10-23
TSCA 
Yes
HS Code 
29221980
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H318Causes serious eye damage

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
PHG0004
Product name
BIS-TRIS
Packaging
1KIT
Price
$1123
Updated
2024/03/01
Sigma-Aldrich
Product number
B4429
Product name
BIS-TRIS
Purity
BioPerformance Certified, cell culture tested, suitable for insect cell culture, ≥98.0%
Packaging
1kg
Price
$1220
Updated
2024/03/01
Sigma-Aldrich
Product number
391335
Product name
BIS-Tris, ULTROL Grade - CAS 6976-37-0 - Calbiochem
Purity
Azwitterionicbufferthatexhibitsonlyaverysmallshiftindissociationcons
Packaging
100g
Price
$179
Updated
2024/03/01
Sigma-Aldrich
Product number
391335
Product name
BIS-Tris, ULTROL Grade - CAS 6976-37-0 - Calbiochem
Purity
Azwitterionicbufferthatexhibitsonlyaverysmallshiftindissociationcons
Packaging
1kg
Price
$898
Updated
2024/03/01
Sigma-Aldrich
Product number
14879
Product name
BIS-TRIS
Purity
BioUltra, ≥99.0% (NT)
Packaging
100g
Price
$244
Updated
2024/03/01
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BIS-Tris Chemical Properties,Usage,Production

Chemical Properties

White/clear crystalline powder

Uses

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Uses

It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.

Uses

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Definition

ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

BIS-Tris Preparation Products And Raw materials

Raw materials

Preparation Products

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BIS-Tris Suppliers

Americas 1 Belgium 4 Canada 3 China 307 Europe 4 France 4 Germany 16 India 22 Italy 1 Japan 5 Poland 1 South Korea 1 Switzerland 3 The Netherlands 3 United Kingdom 18 United States 59 Global 452
Matrix Fine Chemicals GmbH
Tel
--
Fax
--
Email
nfo@matrix-fine-chemicals.com
Country
Switzerland
ProdList
6968
Advantage
58
Sorachim SA
Tel
--
Fax
--
Email
contact@sorachim.com
Country
Switzerland
ProdList
68
Advantage
58
SIGMA-RBI
Tel
--
Fax
--
Country
Switzerland
ProdList
6896
Advantage
91
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View Lastest Price from BIS-Tris manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Bis-Tris 6976-37-0
Price
US $180.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-31
WUHAN FORTUNA CHEMICAL CO., LTD
Product
BIS-TRIS 6976-37-0
Price
US $0.00-0.00/Kg/Drum
Min. Order
1KG
Purity
99%-101%
Supply Ability
500 KG
Release date
2021-05-31
Watson Biotechnology Co.,Ltd
Product
BIS-TRIS 6976-37-0
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20MT
Release date
2024-08-02

6976-37-0, BIS-TrisRelated Search:

4,4'-Methylenedianiline 2-(METHYLSULFONYL)ETHANOL Hydroxyethyl starch Tricine Tris(hydroxymethyl)nitromethane Hydroxyethyl Cellulose Tris(hydroxymethyl)aminomethane Methyl 2-hydroxyethyl cellulose 2-(2-Aminoethylamino)ethanol BUFFER SOLUTION PH = 6,00 (20 °C) (POTASSIUM DIHYDROGEN PHOSPHATE/SODIUM HYDROXIDE) Sodium isethionate 2-(ISOPROPYLAMINO)ETHANOL tert-Butylamine Triethylamine Diethanolamine 2-Amino-1,3-propanediol BIS-TRIS-D19 Hydroxymethyl

  • 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE
  • 2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL
  • 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol
  • Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane
  • Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade
  • Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS
  • Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol)
  • BIS-TRIS BIOULTRA, >= 99.0% (NT)
  • BIS-TRIS, BIOPERFORMANCE CERTIFIED&
  • 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane Bis-tris
  • BIS-Tris, ULTROL
  • Bis-Tris, Cell culture reagent
  • BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE
  • BIS(2-HYDROXYETHYL)IMINO-TRIS(HYDROXYMETHYL)PROPANE
  • BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE
  • BIS(HYDROXYETHYL)IMINO-TRIS-(HYDROXYMETHYL)-METHANE
  • ''BIS-TRIS''
  • BIS-TRIS
  • BIS-TRIS BUFFER
  • 2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILOETHANOL
  • 2,2-BIS-(HYDROXYMETHYL)-2,2',2'-NITRILOTRIETHANOL
  • 2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILOTRIETHANOL
  • 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, 99+%
  • 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, for biochemistry, 99+%
  • Bis[(2-hydroxyethyl)amino]-2-(hydroxymethyl)- 1,3-propanediol
  • 1,3-Propanediol, 2-bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-
  • Bis(2-hydroxyethyl)iminotris(hydroxylmethyl)methane
  • BIS-TRIS,BIOLOGICALBUFFER
  • Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)propane
  • BIS(2-HYDROXYETHYL)-TRIS(HYDROXYMETHYL)METHANE
  • BIS-TRIS (BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE)
  • BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE(BIS-TRIS)
  • Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane >98%
  • Bis(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methane biological buffer
  • bis-tris cell culture tested
  • bis-tris free base
  • bis-tris free base sigmaultra
  • BIS-Tris, ULTROL Grade
  • 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
  • 2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILO- TRIETHANOL, 98+%
  • BIS-TRIS, BIOTECHNOLOGY PERFORMANCE CERT IFIED
  • BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMET L)-METHAN
  • BIS(2-HYDROXYETHYL)IMINO-TRIS(HYDROXYMETHYL)PROPANE （BIS-TRIS）
  • (2-Hydroxyethyl)iMinotris(hydroxyMethyl)Methane(BIS-TRIS)
  • 2-[bis(2-hydroxyethyl)aMino]-3-(hydroxyMethyl)pentane-1,5-diol
  • Bis(2-hydroxyethyl)aMinotris(hydroxyMethyl)Methane&nbsp
  • Bis(2-hydroxyethyl)iMinotris(hydroxyMethyl)Methan
  • BIS-HYDROXYETHYL TROMETHAMINE
  • 2,2-Bis(hydroxyMethyl)-2,2',2''-nitrilotriethanol (BIS-TRIS)
  • BIS-TRIS >99%
  • BIS-TRIS Vetec(TM) reagent grade, >=98%, RNase and DNase free
  • COLOREX CLOSTRIDIUM DIFFICILE 90MM
  • INDICATOR PAPER ROTA PH 1-10 ROLL
  • ROSE BENGAL CHLOR.PENASE 90MM DOUBLE-WR.
  • Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane [Good's buffer component for biological research]
  • 2,2-Bis(hydroxymethyl)-2,2μ,2-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
  • 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, 99+%, for biochemistry
  • 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, for biochemistry